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Magic™ Human CHRM1 U2OS Cell Line with BLA (Beta-Lactamase) Reporter, Reporter Assay (CAT#: S01YF-1222-KX4)

This product is a U2OS GPCR stable cell line. It can be applied in high-throughput drug screening and functional assays.

Product Information

  • Target Protein
  • CHRM1
  • Target Protein Species
  • Human
  • Accession Number
  • NM_000738
  • Host Cell Type
  • U2OS
  • Target Classification
  • GPCR
  • Target Family
  • Acetylcholine (Muscarinic) receptor
  • Target Research Area
  • CNS Research; Cardiovascular Research; Digestive and Renal Research
  • Related Diseases
  • Heart Block; Congenital; Alzheimer Disease

Product Properties

  • Assay Types
  • Reporter Assay
  • Assay Reporter
  • BLA (Beta-Lactamase)
  • Mycoplasma Testing
  • Negative
  • Biosafety Level
  • Level 1
  • Activity
  • Yes
  • Quantity
  • 8x10⁶ cells
  • Form
  • Frozen cells
  • Selective Antibiotic(s)
  • Regular antibiotics active against mycoplasmas, bacteria and fungi.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Incubation
  • 37°C with 5% CO₂
  • Applications
  • Drug screening and biological assays
  • Application Notes
  • Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
  • Use Restrictions
  • These cells are distributed for research use only.
  • Shipping
  • Dry ice
  • Storage
  • Liquid nitrogen

Target

  • Full Name
  • Cholinergic receptor muscarinic 1
  • Introduction
  • The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.
  • GPCR Signaling Pathway
  • The endogenous ligand is acetylcholine. Targeted protein activation can cause binding of Gq to G11 protein which, in turn, activate a phosphatidylinositol-calcium second messenger system. It plays a critical role in Heart Block, Congenital and Alzheimer Disease.
  • Endogenous Ligand
  • Acetylcholine
  • Alternative Names
  • M1; HM1; M1R; muscarinic acetylcholine receptor M1; acetylcholine receptor, muscarinic 1; CHRM1; Cholinergic receptor muscarinic 1

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