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Magic™ Human EDNRB HEK293T Cell Line with BLA (Beta-Lactamase) Reporter, Reporter Assay (CAT#: S01YF-1222-KX62)

Datasheet
This product is a HEK293T GPCR stable cell line. t can be applied in high-throughput drug screening and functional assays.

Product Information

  • Target Protein
  • EDNRB
  • Target Protein Species
  • Human
  • Accession Number
  • NM_000115.1
  • Host Cell Type
  • HEK293T
  • Target Classification
  • GPCR
  • Target Family
  • Endothelin Receptor
  • Target Research Area
  • Cardiovascular Research
  • Related Diseases
  • Waardenburg Syndrome; Type 4A and Abcd Syndrome

Product Properties

  • Assay Types
  • Reporter Assay
  • Assay Reporter
  • BLA (Beta-Lactamase)
  • Mycoplasma Testing
  • Negative
  • Biosafety Level
  • Level 1
  • Activity
  • Yes
  • Quantity
  • 2x10⁶ cells
  • Form
  • Frozen cells
  • Selective Antibiotic(s)
  • Regular antibiotics active against mycoplasmas, bacteria and fungi.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Incubation
  • 37°C with 5% CO₂
  • Applications
  • Drug screening and biological assays
  • Application Notes
  • Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
  • Use Restrictions
  • These cells are distributed for research use only.
  • Shipping
  • Dry ice
  • Storage
  • Liquid nitrogen

Target

  • Full Name
  • Endothelin receptor type B
  • Introduction
  • The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
  • GPCR Signaling Pathway
  • The endogenous ligand is endothelin-1. Targeted protein activation can cause binding of Gi to Go protein which, in turn, cause an inhibition of adenylate cyclase and then decrease of cAMP concentration.
  • Endogenous Ligand
  • Endothelin-1
  • Alternative Names
  • ETB; ET-B; ETB1; ETBR; ETRB; HSCR; WS4A; ABCDS; ET-BR; HSCR2; Hirschsprung disease 2; endothelin receptor non-selective type; endothelin receptor subtype B1; EDNRB; Endothelin receptor type B

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