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Magic™ Human FLAG tagged C5AR1 HEK293T Cell Line, Calcium flux assay (CAT#: S01YF-1022-KX202)

This product is a HEK293T stable cell line. It stably expresses FLAG tagged C5AR1 to provide cell models for research and screening applications.

Product Information

  • Target Protein
  • C5AR1
  • Target Protein Species
  • Human
  • Accession Number
  • NM_001736
  • Protein Tag
  • FLAG-tag at N-terminus
  • Host Cell Type
  • HEK293T
  • Target Classification
  • GPCR
  • Target Family
  • Anaphylatoxin
  • Target Research Area
  • Cancer Research; Inflammation Research
  • Related Diseases
  • Hypersensitivity Reaction Type III Disease; Mast-Cell Sarcoma

Product Properties

  • Assay Types
  • Calcium flux assay
  • Resistance
  • Puromycin
  • Stability
  • Stable for a minimum of 2 months in continuous culture
  • Mycoplasma Testing
  • Negative
  • Biosafety Level
  • Level 1
  • Activity
  • Yes
  • Quantity
  • 2x10⁶ cells
  • Form
  • Frozen cells
  • Culture Medium
  • DMEM, 10% FBS, 1 μg/mL puromycin
  • Selective Antibiotic(s)
  • Regular antibiotics active against mycoplasmas, bacteria and fungi.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Incubation
  • 37°C with 5% CO₂
  • Applications
  • Drug screening and biological assays
  • Application Notes
  • Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
  • Use Restrictions
  • These cells are distributed for research use only.
  • Shipping
  • Dry ice
  • Storage
  • Liquid nitrogen

Target

  • Full Name
  • Complement C5a receptor 1
  • Introduction
  • Enables G protein-coupled receptor activity and complement component C5a receptor activity. Involved in several processes, including complement component C5a signaling pathway; mRNA transcription by RNA polymerase II; and positive regulation of ERK1 and ERK2 cascade. Located in apical part of cell and basolateral plasma membrane. Biomarker of Alzheimer's disease; asthma; chronic obstructive pulmonary disease; rhinitis; and severe acute respiratory syndrome.
  • GPCR Signaling Pathway
  • The endogenous ligand is complement C5a. Targeted protein activation can cause binding of Gi to Go protein which, in turn, cause an inhibition of adenylate cyclase and then decrease of cAMP concentration.
  • Endogenous Ligand
  • Complement C5a
  • Alternative Names
  • C5A; C5AR; C5R1; CD88;C5a anaphylatoxin chemotactic receptor 1;C5a anaphylatoxin receptor;C5a ligand;C5a-R;complement component 5a receptor 1;C5a anaphylatoxin chemotactic receptor;C5a-R;C5aR;CD88;C5AR1

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