Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
With over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications.
Creative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
With deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
Get a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
This product is a HEK293T stable cell line. It stably expresses FLAG tagged C5AR1 to provide cell models for research and screening applications.
Product Information
Target Protein
C5AR1
Target Protein Species
Human
Accession Number
NM_001736
Protein Tag
FLAG-tag at N-terminus
Host Cell Type
HEK293T
Target Classification
GPCR
Target Family
Anaphylatoxin
Target Research Area
Cancer Research; Inflammation Research
Related Diseases
Hypersensitivity Reaction Type III Disease; Mast-Cell Sarcoma
Product Properties
Assay Types
Calcium flux assay
Resistance
Puromycin
Stability
Stable for a minimum of 2 months in continuous culture
Mycoplasma Testing
Negative
Biosafety Level
Level 1
Activity
Yes
Quantity
2x10⁶ cells
Form
Frozen cells
Culture Medium
DMEM, 10% FBS, 1 μg/mL puromycin
Selective Antibiotic(s)
Regular antibiotics active against mycoplasmas, bacteria and fungi.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance. Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line. Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Incubation
37°C with 5% CO₂
Applications
Drug screening and biological assays
Application Notes
Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
Use Restrictions
These cells are distributed for research use only.
Shipping
Dry ice
Storage
Liquid nitrogen
Target
Full Name
Complement C5a receptor 1
Introduction
Enables G protein-coupled receptor activity and complement component C5a receptor activity. Involved in several processes, including complement component C5a signaling pathway; mRNA transcription by RNA polymerase II; and positive regulation of ERK1 and ERK2 cascade. Located in apical part of cell and basolateral plasma membrane. Biomarker of Alzheimer's disease; asthma; chronic obstructive pulmonary disease; rhinitis; and severe acute respiratory syndrome.
GPCR Signaling Pathway
The endogenous ligand is complement C5a. Targeted protein activation can cause binding of Gi to Go protein which, in turn, cause an inhibition of adenylate cyclase and then decrease of cAMP concentration.