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Magic™ Human FLAG tagged LPAR1 RH7777 Cell Line, Calcium flux assay (CAT#: S01YF-1022-KX403)

This product is a RH7777 stable cell line. It stably expresses FLAG tagged LPAR1 to provide cell models for research and screening applications.

Product Information

  • Target Protein
  • LPAR1
  • Target Protein Species
  • Human
  • Accession Number
  • NM_001401
  • Protein Tag
  • FLAG-tag at N-terminus
  • Host Cell Type
  • RH7777
  • Target Classification
  • GPCR
  • Target Family
  • Lysophospholipid
  • Target Research Area
  • Cancer Research; Inflammation Research; Cardiovascular Research
  • Related Diseases
  • Pulmonary Fibrosis; Ovarian Cancer

Product Properties

  • Assay Types
  • Calcium flux assay
  • Resistance
  • Puromycin
  • Stability
  • Stable for a minimum of 2 months in continuous culture
  • Mycoplasma Testing
  • Negative
  • Biosafety Level
  • Level 1
  • Activity
  • Yes
  • Quantity
  • 2x10⁶ cells
  • Form
  • Frozen cells
  • Culture Medium
  • DMEM, 10% FBS, 5 μg/mL puromycin
  • Selective Antibiotic(s)
  • Regular antibiotics active against mycoplasmas, bacteria and fungi.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Incubation
  • 37°C with 5% CO₂
  • Applications
  • Drug screening and biological assays
  • Application Notes
  • Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
  • Use Restrictions
  • These cells are distributed for research use only.
  • Shipping
  • Dry ice
  • Storage
  • Liquid nitrogen

Target

  • Full Name
  • Lysophosphatidic acid receptor 1
  • Introduction
  • The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Many transcript variants encoding a few different isoforms have been identified for this gene.
  • GPCR Signaling Pathway
  • The endogenous ligand is lysophosphatidic acid. Targeted protein activation can cause binding of Gq to G11 protein which, in turn, activate a phosphatidylinositol-calcium second messenger system.
  • Endogenous Ligand
  • Lysophosphatidic acid
  • Alternative Names
  • EDG2; LPA1; VZG1; edg-2; vzg-1; Gpcr26; Mrec1.3; rec.1.3;lysophosphatidic acid receptor 1;LPA receptor 1;LPA-1;endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 2;lysophosphatidic acid receptor Edg-2;ventricular zone gene 1;LPAR1

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