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Magic™ Human KIT (W557_K558del, D816V) BaF3 Cell Line (CAT#: S01YF-0324-KX173)

This product is a BaF3 stable cell line. It stably expresses Human KIT (W557_K558del, D816V) to provide cell models for research and screening applications.

Product Information

  • Target Protein
  • KIT (W557_K558del, D816V)
  • Target Protein Species
  • Human
  • Host Cell Type
  • BaF3
  • Target Classification
  • Kinases/Enzyme
  • Target Family
  • Kinases/Enzyme
  • Target Research Area
  • Cancer Research
  • Related Diseases
  • Piebald Trait; Gastrointestinal Stromal Tumor

Product Properties

  • Assay Types
  • Functional assay and biological assay
  • Stability
  • 16 passages
  • Mycoplasma Testing
  • Negative
  • Biosafety Level
  • Level 1
  • Activity
  • Yes
  • Form
  • Frozen cells
  • Freeze Medium
  • 90% FBS+10% DMSO
  • Culture Medium
  • RPMI-1640+10%FBS
  • Selective Antibiotic(s)
  • Regular antibiotics active against mycoplasmas, bacteria and fungi.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Incubation
  • 37°C with 5% CO₂
  • Applications
  • Anti-proliferation assay and PD assay
  • Application Notes
  • Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
  • Use Restrictions
  • These cells are distributed for research use only.
  • Shipping
  • Dry ice
  • Storage
  • Liquid nitrogen

Target

  • Full Name
  • KIT proto-oncogene, receptor tyrosine kinase
  • Introduction
  • This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
  • Alternative Names
  • KIT; PBT; SCFR; C-Kit; CD117; MASTC; mast/stem cell growth factor receptor Kit; c-Kit protooncogene; p145 c-kit; piebald trait protein; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; soluble KIT variant 1; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; KIT proto-oncogene, receptor tyrosine kinase
  • Gene ID
  • 3815
  • UniProt ID
  • P10721

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