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Magic™ Human MS4A1 BJAB Cell Line with GFP Reporter, Drug screening assay (CAT#: S01YF-1222-KX1)

Datasheet
This product is a stable cell line. It stably expresses Human CD20 to provide cell models for research and screening applications. GFP was fused to the amino terminus of CD20. By transfecting and selecting with G418 and cell sorting, stable transfectants of BJAB cells expressing GFP-CD20 were created.

Product Information

  • Target Protein
  • MS4A1
  • Target Protein Species
  • Human
  • Host Cell Type
  • BJAB
  • Target Classification
  • Immune Checkpoint
  • Target Family
  • Immune Checkpoint
  • Target Research Area
  • Autoimmune Research; Immunology Research
  • Related Diseases
  • Immunodeficiency

Product Properties

  • Morphology
  • Typical lymphoid
  • Assay Types
  • Drug screening assay
  • Assay Reporter
  • GFP
  • Resistance
  • G418
  • Mycoplasma Testing
  • Negative
  • Biosafety Level
  • Level 2
  • Activity
  • Yes
  • Form
  • Frozen cells
  • Selective Antibiotic(s)
  • Regular antibiotics active against mycoplasmas, bacteria and fungi.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Incubation
  • 37°C with 5% CO₂
  • Applications
  • Drug screening and biological assays
  • Application Notes
  • Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
  • Use Restrictions
  • These cells are distributed for research use only.
  • Shipping
  • Dry ice
  • Storage
  • Liquid nitrogen

Target

  • Full Name
  • Membrane spanning 4-domains A1
  • Introduction
  • This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein.
  • Alternative Names
  • MS4A1; B1; S7; Bp35; CD20; FMC7; CVID5; MS4A2; LEU-16; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; membrane-spanning 4-domains, subfamily A, member 1; Membrane spanning 4-domains A1
  • UniProt ID
  • 931

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