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Magic™ Human NT5E (Middle) MC-38 Cell Line (CAT#: S01YF-0123-KX69)

This product is a MC-38 stable cell line. It stably expresses Human NT5E (Middle) to provide cell models for research and screening applications.

Product Information

  • Target Protein
  • NT5E (Middle)
  • Target Protein Species
  • Human
  • Accession Number
  • NM_001204813.2
  • Protein Tag
  • Tag-free
  • Host Cell Type
  • MC-38
  • Target Classification
  • Others
  • Target Family
  • Others
  • Target Research Area
  • Cardiovascular Research; Inflammation Research
  • Related Diseases
  • Calcification Of Joints And Arteries; Periarthritis

Product Properties

  • Morphology
  • Epithelial
  • Assay Types
  • Drug screening and biological assays
  • Resistance
  • Puromycin
  • Mycoplasma Testing
  • Negative
  • Sterility Testing
  • 10 passages
  • Biosafety Level
  • Level 1
  • Activity
  • Yes
  • Quantity
  • 5x10⁶ cells
  • Form
  • Frozen cells
  • Freeze Medium
  • 70% RPMI 1640 + 20% FBS + 10% DMSO
  • Culture Medium
  • RPMI 1640 + 10% FBS + 5µg/mL Puromycin
  • Selective Antibiotic(s)
  • Regular antibiotics active against mycoplasmas, bacteria and fungi.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Incubation
  • 37°C with 5% CO₂
  • Applications
  • Drug screening and biological assays
  • Application Notes
  • Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
  • Use Restrictions
  • These cells are distributed for research use only.
  • Shipping
  • Dry ice
  • Storage
  • Liquid nitrogen

Target

  • Full Name
  • 5'-nucleotidase ecto
  • Introduction
  • The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.
  • Alternative Names
  • NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA; 5'-nucleotidase; 5'-NT; 5'-deoxynucleotidase; IMP-specific 5'-nucleotidase; Purine 5-Prime-Nucleotidase; ecto-5'-nucleotidase; thymidylate 5'-phosphatase

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