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Magic™ Large-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for His-tagged Protein Production (CAT#: MPCFK-3-9)

Magic™ Large-scale Cell-free Protein Expression in Wheat Germ lysate for His-tagged Protein Production Kit is a cell-free protein synthesis system using wheat germ extracts for His-tagged Protein Production. It contains 1 ml optimized and high-performance wheat germ extract for different reaction scales. The lysate removed wheat proteins that would otherwise bind to Ni resins during the purification step. This kit is premixed and allow to setup the transcription and translation reaction by just a few pipetting steps. It's a bilayer format for performing large-scale protein expression reactions.

Product Specifications

  • Lysate Type
  • Wheat Germ lysate
  • Size
  • Different reaction scales
  • Reaction Volume
  • Different reaction scales
  • Expected Yield of Positive Control
  • 20-40 µg/ml
  • Components
  • Wheat Germ Extracts
    40xReaction buffer component 1
    40xReaction buffer component 2
    40xReaction buffer component 3
    40xReaction buffer component 4
    Creatine Kinase
    5×Transcription Reagent
    NTPmix
    RNase Inhibitor
    SP6 RNA Polymerase
  • Storage Conditions
  • Store at -80°C.

Customer reviews and Q&As    

Customer Review

Clear target band

The protein bands were clear, and a large number of proteins were expressed well.
2023.1.16

Q&As

What are the advantages of His-tagged protein production?

Higher purity of purified proteins can be obtained by using His affinity tags.
2023.01.30

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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