Creative Biolabs offers the unparalleled mass sequencing service to profile the B cell receptor (BCR) and T cell receptor (TCR) repertoires. Equipped with world-leading technology platforms and professional scientific staff in the field of immunology, Creative Biolabs can provide high-grade service of quantitative profiling of immune repertoires for small lymphocytes.
Technical Background to Study Small Lymphocytes
Emerging high-throughput next-generation sequencing (NGS) methods for the analysis of the complex structure of T cell receptors and B cell receptors repertoires give a powerful impulse to adaptive immunity studies. These methods allowed for the deep and, to a certain extent, quantitative analysis of the clonotypic composition of BCR and TCR diversity. This enables researchers to further understand the immune repertoires in health and disease, both at the general scale and in diverse lymphocyte subpopulations. Many different technical approaches have been developed to prepare immune repertoire libraries and correct accumulated PCR and sequencing errors. However, essential technical obstacles for performing a truly quantitative analysis still exist. Speciﬁcally, it remains challenging to obtain comprehensive information on the clonal composition of limited small lymphocyte populations, such as antigen (Ag)-speciﬁc, tissue-resident cell subsets, microdissection, or ﬁne needle aspirates. Thus, a powerful approach is required to preserve the original clonotypic composition of immune repertoire.
Quantitative Immune Repertoires Profiling for Small Lymphocytes in Creative Biolabs
To achieve the best high-throughput sequencing capacity, Creative Biolabs has integrated the powerful NGS technology and many other specific techniques into our unique Magic™ platform. Creative Biolabs has developed a robust high-yield NGS approach based on unique molecular identiﬁers (UMIs), which allows qualitative and quantitative proﬁling of immune repertoires on the clonal composition of small lymphocytes. The process consists of cell preparation, PCR ampliﬁcation, sequencing on our Magic™ platform and data analysis. Above all, we employed barcoding of each cDNA molecule using UMIs. This strategy minimizes quantitative biases associated with uneven ampliﬁcation efﬁciency for different Ag receptor genes.
Creative Biolabs has established the high-throughput Magic™ platform for large-scale sequencing services. With years of research and development experience in the field of immunology, Creative Biolabs has accumulated sufficient experience in quantitative profiling of BCR/TCR repertoires for small lymphocytes. We are pleased to offer the best service with the most accurate results for our global customers.
Please contact us for more information and a detailed quote.