Creative Biolabs is able to provide the unique T-cell receptor (TCR) repertoire analysis service for our customers all over the world. With our powerful Magic™ platform, scientists from Creative Biolabs are confident in sequencing CDR regions of the whole four types of TCR chains (α, β, γ, and δ) accurately and comprehensively.
The effective TCR repertoire plays an essential role in the adaptive immune system. TCR, which locates on the T-cell surface, is responsible for interacting with peptide fragments of antigens that bound to major histocompatibility complex (MHC) molecules. In general, most of TCR consists of an α chain and a β chain as a heterodimer, and a minority of TCR is formed by a γ chain and a δ chain as an alternative. Commonly, for a healthy human, α/β TCR accounting for 95% and other 5% is γ/δ TCR. While the proportion between α/β TCR and γ/δ TCR is changeable due to ontogenesis, disease influence or different species. Similar to half of the Fab fragment of an antibody, each TCR chain is composed of a constant and a variable region which also has three complementarity determining regions (CDRs). Meanwhile, the TCR α chain and γ chain are generated by VJ recombination (just like the light chain of an antibody), and the β chain and δ chain undergo VDJ recombination (similar to the heavy chain of an antibody). During this rearrangement mechanism, the nucleotide additions or deletions at the junctions have endowed the TCR repertoire with essential diversity. In this way, the sequencing and analysis of TCR repertoire is a meaningful direction to enhance the understanding of the immune system, especially under different stimulation, such as infections, autoimmune diseases, and cancers, and contribute to discovering new therapeutic agents.
Fig.1 The function and composition of T-cell Receptor.
To achieve the best high-throughput sequencing capacity, Creative Biolabs has integrated the popular next generation sequencing (NGS) technology and other specific techniques into our unique Magic™ platform. This platform can be widely used in large-scale sequencing but also available to be specially designed for certain service. For the sequencing of TCR repertoire, the Magic™ IR-seq platform permits RNA from human or mice as a template to amplify and sequence the complementary determining region (CDR) of whether α, β, γ or δ chain of TCR. Through this excellent platform, our clients can obtain the sequence data of whole covered CDR 3 regions of the interested TCR chain from either human or mice, which has involved most of the hypermutation information.
Besides regular sequencing analysis of the TCR repertoire, we also provide a series of custom analysis options including but not limit to:
Features of Magic™ platform for repertoire analysis
Creative Biolabs is a long-term expert in the field of immunology who has extensive experience and advanced technology platform for NGS sequencing. Our scientists have accomplished over hundreds of sequencing projects and accumulated sufficient knowledge and understanding of the adaptive immune system. We are pleased to offer the best service with the most accurate sequencing results for our global customers.