Creative Biolabs is a leading service provider of monoclonal antibody production from various species. Our scientists are pleased to introduce our novel technology of generating monoclonal antibodies in the donkey. Combined with the advanced phage display platform established by Creative Biolabs, the monoclonal donkey antibodies could be developed in an efficient and cost-effective manner.
Nowadays, immunization of laboratory animals is a routine procedure for the induction of humoral or cellular immune responses. Upon injection of an antigen, donkey will generate multiple antibodies to many epitopes. A single clone of B-cells will produce antibodies to only a single epitope. While this B-cell divides, the resulted numerous B-cells will all produce this single type of antibody targeting a specific epitope. Multiple clones of B-cells may produce antibodies to the same epitope sometimes. Compared to polyclonal antibodies, the donkey monoclonal antibodies can be a unique source for certain kinds of research. They will take similar advantages to the monoclonal antibody from other species, such as high specificity and stable quality from batch to batch.
As a classical antibody development method, hybridoma, however, is not easy for donkey monoclonal antibody generation in general because of the lack stable fusion partner. Therefore, Creative Biolabs has developed an advanced phage display platform for monoclonal antibody production. The antibody library of the immunized animal will be constructed with a diversity of ~1010. In addition, the appropriate biopanning strategy is also a key step for selecting antibodies with high affinity and specificity. Scientists are able to carry out several different methods, such as solid phase screening, solution-sorting, and cell-based screening, to enrich the high-affinity binders successfully. Moreover, the resulted antibody targets could be further engineered with our affinity maturation and humanization services.
However, not all the protein could generate antibodies effectively, for example, some membrane proteins like G protein-coupled receptors (GPCR) are extremely hard to generate specific antibodies. In addition, these targets often have low protein expression levels. Creative Biolabs has developed an MPAT™ platform for this type of challenging targets. DNA immunization service, which is the perfect strategy for membrane protein specific antibody production, is also available in Creative Biolabs.
Besides donkey monoclonal antibody production service, Creative Biolabs also provides monoclonal antibodies from various types of animals, including but not limited to:
As a well-recognized antibody related services provider, Creative Biolabs has accomplished thousands of antibody services for our global clients. With years of experience, our scientists are confident in producing the best monoclonal antibodies with high affinity and specificity to satisfy our customers’ requirement. The antibodies derived from various species are essential in the investigation of immunotherapies. Scientists of Creative Biolabs could offer one-stop services from animal immunization, library construction and screening to antibody production to develop the most specific antibody candidates for your goals.