ImmunoArrayᵀᴹ Extractable Nuclear Antigen (ENA) and Antinuclear Antibody (ANA) Detection Service (32 Antigens) (CAT#: NA-0726-FY25)

Developed by Creative Biolabs, the ENA and ANA Detection Service leverages an advanced, high-sensitivity protein microarray platform to profile antibody signatures against 32 thoroughly vetted, high-purity nuclear antigens. By locking these proteins in their natural 3D configuration across three concentration gradients on the slide surface, this service enables comprehensive, reproducible antibody data collection from just a 5 μL biofluid sample. It serves as a robust tool for translational medical research, allowing investigators to identify early clinical biomarkers, study rheumatic disease pathogenesis, and formulate multi-parametric diagnostic and prognostic models.

Specifications
Size16-sample format per slide; features multiplex capability detecting>120 targets per well (encompassing the 32 disease-related ENA/ANA proteins printed in 3 different concentrations/replicates).
Throughput LevelHigh-throughput (processes 16 samples per slide simultaneously, with support for parallel operations across multiple slides).
Protein Count32 types of highly relevant ENA and ANA antigen proteins.
Proteome CoverageTarget-specific panel consisting of 32 pure nuclear antigens most strongly associated with Systemic Autoimmune Rheumatic Diseases (SARD).
Arraying TechnologyHigh-sensitivity antigen microarray technology (protein microarray) where nuclear antigens are precisely immobilized onto a 3D coated surface in 3 different concentrations/replicates; molecular interactions are captured via fluorescently labeled secondary antibodies and quantified using a fluorescence scanner.
Quality ControlEach array integrates internal Ig and anti-Ig control spots to ensure rigorous data normalization and reliable quantitative comparison across microarrays.
Features3-Concentration Replicate Printing: Each antigen is spotted at 3 concentration levels to optimize the antigen-antibody binding ratio, yielding excellent sensitivity and high reproducibility. Dual-Isotype/Subtype Detection: Uses multi-isotype fluorescent secondary antibodies to detect two different antibody classes or subclasses simultaneously in a single run. High Multiplexing: Screens>120 targets concurrently per well, far outperforming traditional single-target ELISA assays. Ultra-low Sample Volume: Requires only 5 μL of serum or other body fluids to minimize sample depletion. High Sensitivity & Wide Dynamic Range: Operates within a broad signal dynamic range from 1 to 65000. Cost-Effective: Delivers comprehensive systemic profiling at less than 1/10 of the cost of traditional ELISA kits. Semi-Quantitative: Supports relative quantification based on standard curves.
Application FieldsSystemic Autoimmune Rheumatic Diseases (SARD): High-efficiency screening and profiling of key biomarkers for the identification and clinical tracking of Systemic Lupus Erythematosus (SLE), Systemic Sclerosis/Scleroderma (SSc), Sjögren's syndrome (SS), and Mixed Connective Tissue Disease (MCTD). Other Autoimmune Disorders: Profiling of broader systemic conditions including Rheumatoid Arthritis (RA), diabetes, polymyositis, dermatomyositis, and allergic diseases. Translational & Clinical Research: Scale-driven analysis of immune responses across infectious diseases, brain nerve disorders/neurological impairments, oncology (cancer biomarkers/immunotherapy toxicity monitoring), vaccine R&D/post-vaccination safety monitoring (e.g., SARS-CoV-2, RSV, influenza), organ transplantation evaluation, drug screening, and clinical trials.
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