ImmunoArrayᵀᴹ Glycosphingolipid (GSL) Glycan Microarray (CAT#: NA-0726-FY36)

The Glycosphingolipid (GSL) Glycan Microarray is a high-sensitivity carbohydrate-binding matrix engineered to systematically map interactions with cell-surface glycolipid sugar headgroups. Displaying 58 structurally distinct synthetic GSL variants-encompassing Globo-, Lacto-, Neolacto-, and Ganglio-type arrangements-immobilized across an 8- or 16-subarray configuration, this high-throughput tool resolves target binding specificities from minimal sample volumes. By presenting complex neutral, sialylated, or sulfated sugar branches without their hydrophobic ceramide anchors, this platform enables translational medicine researchers to decipher how membrane-associated carbohydrate codes dictate cell-to-cell communication, viral/bacterial docking, and neurodegenerative or malignant disease trajectories.

Specifications
SizeMulti-array slide format featuring 8 or 16 identical sub-arrays per chip, enabling parallel multi-sample profiling. It accommodates a total of 58 synthetically prepared GSL glycan targets plus controls.
Throughput LevelHigh-throughput (enables the parallel analysis of 8 to 16 samples on a single chip, yielding reliable ligand-binding readouts within a few hours).
Arraying TechnologyMultiplex glycan microarray technology where 58 distinct synthetic GSL glycan moieties are orderly printed onto the sub-arrays of a solid slide surface; molecular binding interactions (such as the Wisteria floribunda agglutinin [WFA] lectin binding model resolved via biotin-streptavidin-Cy3 chemistry) are captured and measured using a microarray scanner at a 532 nm wavelength.
Quality ControlIntegrated positive controls are embedded within each sub-array layout to demonstrate expected target-ligand binding signals (such as verification of signature GM2 and GD2 signaling pathways), ensuring accurate printing alignment and overall assay validity.
Features58 Synthetic GSL Core Structures: Features a specialized library of 58 structurally defined synthetic GSL glycan headgroups to bypass natural lipid isolation complexities. High Sensitivity & Specificity: Highly optimized to identify subtle variations in configuration types (Globo, Lacto, Neolacto, Ganglio) and terminal functional changes (sialylation/sulfation). Flexible 8 or 16 Sub-Array Format: Designed with 16 or 8 identical testing zones per slide to facilitate streamlined multi-variable comparative screening. Ultra-low Sample Volume: Employs a simplified, highly efficient micro-assay format requiring only minimal biological sample sizes. Customization & Service Support: Fully customizable to meet individual research goals, with professional companion assay screening and detection services available upon request.
Application FieldsBinding Specificity Evaluation: Assessing the precise carbohydrate-binding preferences of GSL-interacting host proteins (such as WFA lectins) and target antibodies. Pathogen Host-Entry Research: Investigating complex structural interactions between defined cell-surface GSL chains and viruses or bacteria that target lipid-raft glycans during host invasion. Onco-Immunology & Metabolic Profiling: Investigating abnormal GSL glycan profiles that serve as malignant hallmarks promoting cancer cell survival, mapping tumor-associated carbohydrate antigens, and studying GSL dysregulation tied to neuroinflammation in Parkinson's disease. Interactome & Cellular Profiling: Exploring binding activities with diverse biological targets including whole cells, cell lysates, serum, and vesicles.
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For Research Use Only | Not For Clinical Use

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