ImmunoArrayᵀᴹ Sialylated Glycan Microarray (CAT#: NA-0726-FY32)

The Sialylated Glycan Microarray is a high-sensitivity carbohydrate-binding matrix engineered to systematically map interactions with terminal sialic acid configurations. Displaying 50 structurally distinct N-glycans-including 44 paired Neu5Gc/Neu5Ac variants and 6 poly-LacNAc extended lines-immobilized across an 8- or 16-subarray configuration, this high-throughput tool resolves target binding specificities from minimal sample volumes in just a few hours. Serving as a powerful tool for functional glycomics, this platform enables researchers to decode how hypersialylation patterns dictate immune checkpoint inhibition, host-pathogen engagement, and chronic inflammatory disease trajectories driven by dietary or tumor-associated sugar alterations.

Specifications
SizeMulti-array slide format featuring 8 or 16 identical sub-arrays per chip, allowing simultaneous multi-sample profiling. It accommodates a total of 50 structurally defined sialylated glycan targets plus controls.
Throughput LevelHigh-throughput (enables the parallel analysis of 8 to 16 samples on a single chip, yielding reliable ligand-binding readouts within a few hours).
Arraying TechnologyMultiplex glycan microarray technology where 50 distinct sialylated structures are orderly printed onto the sub-arrays of the slide surface; molecular binding interactions (such as the Sambucus nigra lectin [SNA] binding model resolved via biotin-streptavidin-Cy3 chemistry) are captured and quantified using a microarray scanner at a 532 nm wavelength.
Quality ControlIntegrated positive controls and labels are embedded within each sub-array layout to demonstrate expected binding signals (such as linkage-specific α-2,6 sialic acid alignment markers), ensuring printing accuracy and assay validity.
FeaturesPaired Sialic Acid Screening: Features unique structural pairs of human-derived Neu5Ac and dietary/non-human Neu5Gc to detect precise discrimination in immune recognition. High Sensitivity & Specificity: Highly optimized to identify subtle variations in terminal sialylation, poly-LacNAc extensions, and linkage types. Flexible 8 or 16 Sub-Array Format: Engineered with 16 or 8 identical testing zones per slide to facilitate parallel multi-sample processing. Ultra-low Sample Volume: Employs a simplified, highly efficient micro-assay format requiring only minimal biological sample sizes. Customization & Service Support: Fully customizable (allowing users to select specific glycan compositions for custom microarray formats) and available as a comprehensive analytical testing service.
Application FieldsBinding Specificity Evaluation: Assessing the precise binding preferences of sialic acid-interacting proteins (such as Siglecs or lectins) and target antibodies (including anti-Neu5Gc autoantibodies). Pathogen Host-Entry Research: Investigating complex structural interactions between defined sialylated chains and viruses or bacteria that utilize terminal sialic acids as cell-entry receptors. Onco-Immunology & Inflammation Profiling: Studying hypersialylation mechanisms in tumor microenvironments, immune checkpoint evasion (e.g., Siglec-7/-9 interactions on NK and T cells), and chronic dietary-induced inflammation (cardiovascular disease, thyroiditis, and cancer). Interactome & Cellular Profiling: Exploring binding activities with diverse biological targets including whole cells, cell lysates, serum, and vesicles.
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