Omni-Hybridoma™ Technology

We have established an Omni-Hybridoma™ platform, in which newly fused hybridoma cells are subcloned, grown and selected using an agarose-based semi-solid medium containing growth factors, B-cell stimulators and medium supplements optimized for the growth of single hybridoma cells. This revolutionary platform ensures that a large number of hybridoma clones can be selected after each cell fusion.

In traditional hybridoma plating methods, multiple rounds of limiting dilutions are required to obtain monoclonal hybridoma clones. A serious problem is that some hybridoma clones overgrow others prior to cloning. Often, these faster growing cell lines do not synthesize antibodies, resulting in a failure to obtain the best antibody-producing hybridoma clones. Also, it is extremely labor-intensive and time-consuming to single-cell clone a hybridoma clone by performing multiple rounds of limited dilutions, thus making it difficult to isolate a large number of hybridoma clones from each cell fusion.

The Omni-Hybridoma™ platform is designed to select and clone hybridoma cell clones immediately after fusion. This eliminates the possibility of overgrowth of potentially valuable slow-growing clones by fast-growing clones. In this platform, hybridoma colonies are clonal from the start; the number of clones to be screened for the secretion of a specific antibody is minimized because all daughter cells are found in the same colony. Most importantly, since HAT selection and cloning of hybridomas are performed simultaneously in a single step, large numbers of hybridomas can be selected and tested. In fact, more than 1,500 hybridoma clones can be single-cells cloned in ten 15cm dishes after a single fusion, thus significantly increasing the number of candidate hybridoma clones that are specific for the target immunogen.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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