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Organic Molecule Binding Peptides Identification Service

With years of research and development experience in the field of phage display, Creative Biolabs has a group of scientists who own the distinguished phage display technologies. Powered with various types of in-house phage display peptide libraries, Creative Biolabs is able to offer the most comprehensive services and products based on our phage display platform. Our seasoned scientists are pleased to share expertise and experience in phage selection of organic molecule binding peptides with our global clients.

Schematic diagram showing our biomimetic approach to develop selective coatings for gas-phase explosive molecules (Jaworsk et al. 2008).
Fig. 1 Schematic diagram showing our biomimetic approach to develop selective coatings for gas-phase explosive molecules (Jaworsk et al. 2008).

The Background of Organic Molecules as Substrates of Phage Display

Small organic molecules target for phage display is more challenging than synthetic or natural polymers. In some cases, small organic molecules can be directly used as substrates for phage display in form of solid crystals. In many other cases, however, the molecules are immobilized on spherical beads or on planar substrates. Small organic molecules like fluorophores, including Texas Red, Oregon Green 514 and fluorescein, were first used as targets for phage selection. Some research groups have demonstrated the feasibility of using peptides identified by phage display for the selective detection of explosives molecules, such as 1,3,5-trinitrobenzene (TNB), 2,4-dinitrotoluene (DNT) and 2,4,6-trinitrotoluene (TNT). Besides above, phage display has also been utilized for the identification of organic molecule binding peptides for potential sensing applications or surface patterning. Octyltrimethoxysilane (OTMS) binders have been used for patterning OTMS-containing silicon surfaces via microcontact printing or photolithography. Peptides have also been identified for selective recognition of two different polychlorinated biphenyl’s (PCB’s), which are known to be carcinogenic and can accumulate in tissue.

Detection of gaseous 2,4-dinitrotoluene (DNT) using a DNT-binding peptide coupled to a gold surface via a flexible oligoethylene glycol (OEG) linker (Günay and Klok 2015).Fig. 2 Detection of gaseous 2,4-dinitrotoluene (DNT) using a DNT-binding peptide coupled to a gold surface via a flexible oligoethylene glycol (OEG) linker (Günay and Klok 2015).

Creative Biolabs has developed a variety of strategies to identify peptides that can selectively bind to different small organic molecules. Numbers of sub-libraries with extra mutagenesis of the identified phage clones can be constructed for further selection of clones with higher affinity than the originally selected ones.

Small Organic Molecules as Substrates for Peptide Binder Identification

Texas Red PCB’s
Oregon Green 514 Thiamethoxam
Fluorescein OTMS
TNT Naphthalene
TNB Trinitrotoluene
DNT 2, 4-Dinitrotoluene

For over a decade of experience in phage display technology, Creative Biolabs is dedicated to providing our customers with high-quality services. Our scientists can offer high-quality customized services in organic molecule binding peptides identification by adjusting protocols to meet every unique requirement. For more detailed information, please feel free to contact us or directly send us an inquiry.

Reference

  1. Günay, K.A.; Klok, H.A. Identification of soft matter binding peptide ligands using phage display. Bioconjug Chem. 2015, 26(10):2002-15.



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