Introduction of P2RY13
P2RY13, also known as purinergic receptor P2Y13, purinergic receptor P2Y, GPR86, GPR94, FKSG77, GPCR1, or SP174, is an approximately 41 kDa G protein-coupled receptor (GPCR) which is composed of 354 amino acids. GPCR plays a role in cell communication and is characterized by an intracellular C terminus, seven transmembrane regions, and an extracellular N terminus. P2RY13 is a receptor for ADP and in humans, encoded by the P2RY13 gene mapping at the chromosome 3q25.1. It is known to be activated by ADP and probably plays a role in the hematopoiesis and immune system. This receptor is strongly expressed in the spleen and adult brain, while at lower levels in placenta, liver, lung, thymus, uterus, stomach, testis, spinal cord, small intestine, fetal brain, and adrenal gland.
|Basic Information of P2RY13|
|Protein Name||P2Y purinoceptor 13|
|Aliases||P2Y13, P2Y purinoceptor 13, G-protein coupled receptor 86, G-protein coupled receptor 94|
|Organism||Homo sapiens (Human)|
Function of P2RY13 Membrane Protein
At present, there are eight nucleotide P2Y receptors are known in mammalian, including P2RY1, P2RY2, P2RY4, P2RY6, P2RY11-14. The distribution of P2Y receptors in diverse tissues indicates that they accomplish key roles in certain physiological and pathological situations. P2RY13 as one of the latest P2Y receptors identified recently is a new member of the Gi-coupled P2Y receptor subfamily that can respond to ADP, along with P2RY12 and P2RY14. Pharmacology analysis draws attention to this novel ADP receptor, which has a pharmacological overlap with P2RY12 receptors and is equipped with unique features and functions. The P2RY12, 13, 14 genes all reside on the human chromosome 3q25.1 and their high-level sequence homology supports their evolutionary origin by gene duplication. P2RY13 is versatile in its signaling, extending beyond the normal signaling of a Gi-coupled receptor. It not only can couple to various G proteins but also can trigger a number of intracellular pathways correlated with the activation of mitogen-activated protein kinases (MAPKs) and the phosphatidylinositol 3-kinase axis.
Fig.1 Schematic representation of the intracellular cascades triggered by 2MeSADP (activating both P2Y1 and P2Y13 receptors) stimulation in rat cerebellar astrocytes. (Pérez-Sen, 2015)
Application of P2RY13 Membrane Protein in Literature
The result suggested that P2Y13 receptor-activated microglia constitutively moderated hippocampal neurogenesis. Here, selective P2Y13R antagonists were able to promote neurogenesis in pathological conditions related to impaired hippocampal neurogenesis.
Intrathecal MRS2211 generated an anti-nociceptive effect during early-stage diabetic neuropathic pain (DNP). A possible mechanism implicated in MRS2211-induced analgesia was that blocking P2Y13 receptor can down-regulate expressions of IL-1β and IL-6, which later inhibited the activation of JAK2/STAT3 signaling pathway.
In summary, this paper showed that microglia control P2Y1 and P2Y12 receptor-mediated astroglial proliferation via a P2Y12 and P2Y13 receptor-mediated mechanism alternative to the IL-1β suppressive pathway that might implicate the contribution of the cytokines IL-1α and TNF-α.
This article summarized their current understanding of the elusive receptor, not only at the molecular and pharmacological level but also in its signaling properties and specific functions, beneficial to clarify the role of P2Y13 receptors in pathological circumstances.
The review found that MRS2211 can attenuate ADP-mediated raising in [Ca2+]i in glial cells from cultures at two stages, which suggested the presence of P2Y13 receptors coupled to calcium mobilization in proliferating glial progenitors in culture.
P2RY13 Preparation Options
To gain a naturally functional target protein, we have established multiple reconstitution formats and active forms for membrane proteins. Our versatile Magic™ membrane protein production platform can provide global clients a lot of flexible options and aid them to select an optimal match for their special purposes. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-P2RY13 antibody development services.
Herein, Creative Biolabs as a world-class producer in protein market has successfully achieved quantities of membrane protein preparations. We would like to tailor one-stop and self-identity protein services to suit clients’ needs. Please feel free to contact us for more details.