In recent years, engineering of pH-sensitive biomolecules has been of increasing interest due to a large area of potential applications. Powered by our advanced phage display platform, Creative Biolabs has developed a series of tailored strategies for pH-sensitive biomolecules identification. Our scientists can provide high-quality pH-sensitive biomolecules discovery service for all our global customers.
The affinity and specificity of interactions between proteins and proteins or proteins and antibodies can be regulated by external pH. The sensitivity of binding affinity to pH plays an important role in biological processes. For example, maternal immunoglobulin G (IgG) binds the neonatal Fc receptor with high affinity at pH 6.0 and weakly at pH 7.4. This pH sensitivity of binding facilitates transcytosis of maternal IgG across fetal and neonatal tissues and is critical for imparting passive immunity to the fetus before a functional immune system is developed. Researchers have demonstrated that a longer half-life was observed in antibodies with lower pI in variable regions. The introduction of pH-sensitive binding activity can be also used to increase the potency of therapeutic proteins or peptides. Therapeutic proteins or peptides are engineered to lose binding to their target receptor in the acidic environment of the endosome (pH 6.0), which can escape endosomal degradation but result in increased half-life of the protein in the extracellular space. This paradigm has been used to engineer pH-sensitive mutants. Introduction of one or more histidine residues in the binding interface is commonly used to engineer pH sensitivity of binding. The protonation of the histidine side chain changes at lower pH, thereby altering the electrostatic interactions involved in binding, and leads to a change in binding affinity. Phage display has also been used to efficiently screen a combinatorial library of histidine mutants to identify a pH-sensitive single domain antibody.
There is growing interest in the fast and robust engineering of pH-sensitive biomolecules. In terms of the extensive experience in phage display technology, Creative Biolabs has developed a series of strategies for isolation of pH-sensitive biomolecules based on our diverse phage display libraries. Combined with Magic™ Sequencing, pH-sensitive biomolecules in the enriched sublibrary can be identified in a time-saving manner.
Diverse phage display libraries are available:
Based on our powerful phage display platform, Creative Biolabs can provide a series of pH-sensitive biomolecules discovery service including but not limited to:
Integrate with our advanced phage display platform and various types of phage display libraries, Creative Biolabs is able to offer the most comprehensive services for pH-sensitive biomolecules discovery. We are confident to provide top-quality service to facilitate our customers’ development.
Please inquire us for more information and a detailed quotation.