Consistently devoted to accelerating the development pace of antibody technology, Creative Biolabs has equipped with world-leading technology platforms and professional scientific staff. Our scientists proudly introduce our unique service of pH-sensitive single-chain variable fragment antibody (scFv) discovery using our cutting-edge phage display platform. Creative Biolabs is pleased to offer our clients pH-sensitive scFvs with the best quality and most competitive price.
scFv consists of the variable regions of the heavy (VH) and light chains (VL) of immunoglobulins, which are connected with a short linker, about 25 amino acids. The linker is usually rich in glycine for flexibility, as well as serine or threonine for solubility. Moreover, one of the advantages cannot be ignored is that scFvs can be expressed with high productivity in bacteria cell cultures such as E. coli. Since its introduction three decades ago, phage display technology has been extensively tailored, and it is still the most important molecular evolution technology available. High-quality affinity scFvs isolated by phage display technology has been widely used in research, proteome-scale experiments and therapeutic intervention. pH-sensitive scFvs have been engineered to release their target at a slightly acidic pH, which makes them suitable for clinical as well as biotechnological applications.
In the field of phage display technology, Creative Biolabs has accumulated extensive experience. Our scientists combined Magic™ platform with phage display technology to screen clones and determine whether a greater number of clones can be identified. It largely improved the selection of pH-sensitive antibodies by detecting low abundant clones with fewer rounds of selection. Creative Biolabs has also developed a variety of specific selection strategies for isolation of pH-sensitive scFv. By screening our custom immune, naïve or synthetic phage display libraries enriched in histidine, we can rapidly isolate pH-sensitive scFv directed against theoretically any biological target.
Key advantages of pH-sensitive scFv generated by phage display platform including but not limited to:
Creative Biolabs is pleased to share our cutting-edge technology and extensive expertise in pH-sensitives scFv to facilitate our clients’ research and project development. We can offer high-quality customized services by adjusting protocols to meet the most specific requirements of our global customers.
For more detailed information, please feel free to contact us or directly send us an inquiry.