Polyglutamylation Specific Antibody Discovery Service

Background Antibody Types Discovery Strategy Types of PTM Why Choose Us? FAQs

Post-translational modification of proteins represents a complex procedure that creates significant obstacles for researchers focused on disease studies and pharmaceutical development. Creative Biolabs' development service for polyglutamylation-specific antibodies overcomes existing limitations to enable researchers to study this modification more efficiently.

Background

The post-translational modification process known as polyglutamylation occurs through catalysis by enzymes belonging to the polyglutamylase family (TTLL enzymes). The human body primarily exhibits this modification in α-tubulin and β-tubulin molecules. The modification process involves covalent attachment of glutamate polymers to the γ-carboxyl group of the target glutamate residue which becomes crucial for numerous cellular functions, including microtubule-associated carrier transport and cytoskeletal dynamics. Studies have demonstrated that abnormal polyglutamylation processes are a significant feature in neurodegenerative diseases. Protein function depends on precise control through glutamate chain addition and removal and disruption of this regulation can cause disease. Through our customized service researchers can discover polyglutamylation specific antibodies which serve as essential tools for disease investigations and theoretical studies.

Fig. 1 Tubulin heterodimer consisting of α- and β-tubulin. (OA Literature) Fig.1 Tubulin heterodimer consisting of α- and β-tubulin with various posttranslational modifications and representative enzymes.¹

Antibody Types

Creative Biolabs creates monoclonal and polyclonal polyglutamylation-specific antibodies from rabbit, mouse, and sheep hosts. You can obtain our polyglutamylation-specific antibodies in multiple formats.

Site-Specific Anti-Polyglutamylation Antibodies: These antibodies bind to specific polyglutamylation sites on proteins which enables them to detect and measure anti-polyglutamylation modifications with precision.
Pan Anti-Polyglutamylation Antibodies: These antibodies have the capability to detect various polyglutamylated protein forms and they target glutamic acid side chains of diverse lengths.

Discovery Strategy

Creative Biolabs implements three main methods for discovering polyglutamylation-specific antibodies tailored to our worldwide clients.

Monospecific Anti-Polyglutamylation Polyclonal Antibody Production: The traditional method requires immunizing host animals with a particular antigen to produce polyclonal antibodies that target polyglutamylated proteins.

Fig. 2 General process of monospecific anti-polyglutamylation polyclonal antibody production. (Creative Biolabs Original)

Phage Display Strategy for Anti-Polyglutamylation Monoclonal Antibody Discovery: Through phage display, an advanced technique enables researchers to select antibodies with high affinity toward specific polyglutamylated targets by utilizing bacteriophages to present a broad antibody library.

Fig. 3 General process of phage display for anti-polyglutamylation monoclonal antibody discovery. (Creative Biolabs Original)

Hybridoma Strategy for Anti-Polyglutamylation Monoclonal Antibody Discovery: The traditional hybridoma technique creates fused cells from antibody-producing B cells and myeloma cells to generate hybridomas for antibody selection.

Fig. 4 General process of hybridoma for anti-polyglutamylation monoclonal antibody discovery. (Creative Biolabs Original)

Types of PTM

We provide polyglutamylation specific antibodies and antibodies targeting a variety of PTMs, as shown in the table below.

Why Choose Us?

Creative Biolabs stands as your preferred partner for polyglutamylation-specific antibody discovery because we provide:

High-Affi™ Technology: We use proprietary technology to produce antibodies that demonstrate exceptional affinity and specificity.
Comprehensive Service Portfolio: Our comprehensive service offerings extend from antigen design through antibody production and characterization.
Expertise: Our team possesses extensive expertise in PTM biology with a specific focus on polyglutamylation.
Customization: Our services adapt to fit the distinct research requirements of each client.
Published Data: Published data demonstrates our antibody development services' effectiveness, which backs up our proven successful track record.

FAQs

1. What is the role of polyglutamylation-specific antibodies?

Our antibodies deliver the necessary specificity and sensitivity to detect and quantify polyglutamylation which produces reliable and significant research outcomes. Reach out for a consultation about your specific application needs to understand how we can customize our antibodies to optimize your experimental results.

2. Can you use my antibodies across multiple applications such as Western blotting, IHC, and ELISA?

Our antibodies demonstrate high performance across multiple application settings. Our team provides comprehensive validation data and customized protocols upon request.

3. How do your antibody discovery methods provide benefits when compared to alternative approaches?

Through our multiple approaches, like phage display and hybridoma technology and our High-Affi™ Technology, we are able to produce antibodies that exhibit both superior quality and optimal characteristics. Our team would be delighted to conduct a consultation to identify the most effective approach for your project.

4. How should I decide between a site-specific and pan anti-polyglutamylation antibody for my research needs?

You can base your selection on the specific objectives of your research. Site-specific antibodies excel in modification analysis at specific sites, whereas pan antibodies serve as effective tools for broad detection of polyglutamylated proteins. Our specialists will assist you in choosing the optimal antibody once you reach out to us.

5. What support services will I receive after buying your antibodies or using your services?

We deliver full customer support, which encompasses both technical assistance and troubleshooting services. We are committed to ensuring your success. Contact us now to discover the details of our support services.

Creative Biolabs delivers an all-encompassing solution for discovering antibodies that target polyglutamylation. Our services furnish both the essential tools and specialized knowledge you need to push your research forward. Please reach out to us whenever you require information or assistance.

Reference

Ruse, Cristian I., Hang Gyeong Chin, and Sriharsa Pradhan. "Polyglutamylation: biology and analysis." Amino Acids 54.4 (2022): 529-542. Distributed under Open Access license CC BY 4.0, without modification.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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