Protease-neutralizing Antibody Discovery

Protease-neutralizing Antibody DiscoveryCreative Biolabs is a well-recognized leader in the field of antibody development. Based on our advanced phage display technology, our scientists are specialized in the discovery and production of antibodies with high specificity and affinity. Our scientists have developed a novel genetic strategy to isolate antibody-based inhibitors of protease from phage display libraries. We are pleased to use our cutting-edge technologies and extensive experience to offer the best service and the most qualified protease-neutralizing antibody to satisfy each demand of our customers.

The Properties of Protease

Proteases always perform proteolysis, protein catabolism by hydrolysis of peptide bonds and they have evolved multiple times. Different classes of protease can perform the same reaction by completely different catalytic mechanisms. Proteases are involved in digesting long protein into shorter fragments by cutting the peptide bonds. They exist in all organisms, from prokaryotes to eukaryotes to viruses. These enzymes are involved in a multitude of physiological reactions. Proteases not only can break specific peptide bonds depending on the amino acid sequence of a protein, but also break down a complete peptide to amino acids. The activity can abolish a protein's function or digest it to its principal components. Proteases can be an activation of a function, or it can be a signal in a signaling pathway.

The structure of a protease (TEV protease) complexed with its peptide substrate in black with catalytic residues in red (Shafee 2014). Fig.1 The structure of a protease (TEV protease) complexed with its peptide substrate in black with catalytic residues in red (Shafee 2014).

Protease-neutralizing Antibody Discovery Service in Creative Biolabs

Creative Biolabs has developed a novel bacterial genetic strategy to isolate protease-neutralizing antibody from phage display libraries. Our strategy is based on the concerted co-expression of a reporter gene, recombinant protease and fusion-stabilized antibodies (scFv/Fab/VHH) in Escherichia coli. The reporter system has been constructed by inserting a short peptide corresponding to the cleavage site of the protease into a permissive site of the enzyme β-galactosidase. Once isolated, the inhibitors will be validated by an enzyme-linked immunosorbent assay and in vitro catalysis assays.

Key Advantages of Our Protease-neutralizing Antibody Discovery Service Including but Not Limited to:

Creative Biolabs is a leading service provider that focuses on antibody development. Combining with phage display technology, our bacterial genetic strategy has been widely used for protease-neutralizing antibody discovery, such as hepatitis C virus (HCV) serine protease, etc. We are proud to offer our clients a series of antibody discovery services with the best quality and the most competitive price.

Please contact us for more information and a detailed quote.


  1. Shafee, T. Evolvability of a viral protease: experimental evolution of catalysis, robustness and specificity (Doctoral dissertation, University of Cambridge). 2014.
  2. Gal-Tanamy, M.; et al. HCV NS3 serine protease-neutralizing single-chain antibodies isolated by a novel genetic screen. Journal of molecular biology. 2005, 347(5).

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