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Recombinant Human Macrophage Inflammatory Protein 5 /CCL15

Recombinant Human Macrophage Inflammatory Protein 5 /CCL15 CRP08107 1mg Please Inquiry
Product Detail
Product Overview
Recombinant Human MIP-5/CCL15 produced in E. coli is a single, non-glycosylated polypeptide chain containing 92 amino acids and having a molecular mass of 10.1 kDa.
Fully biologically active when compared to standard. Determined by its ability to chemoattract human T lymphocytes using a concentration range of 1.0 -10.0 ng/ml.
Less than 1EU/mg of rHuMIP-5/CCL15 as determined by LAL method.
>97% by SDS-PAGE and HPLC analyses.
This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Antigen Description
CCL15, a new human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 amino acids that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1γ, respectively. The gene for MIP-5 is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-5 is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.
chemoattractant activity;chemokine activity;heparin binding;heparin binding;signal transducer activity.
Gene ID
LKN1;NCC3;SY15;HCC-2;Lkn-1;MIP-5;NCC-3; SCYL3;MIP-1d;SCYA15;HMRP-2B;chemokine (C-C motif) ligand 15; CCL15_HUMAN; MIP5; C-C motif chemokine 15 [Precursor]; Small-inducible cytokine A15;Macrophage inflammatory protein 5;MIP-5;Chemokine CC-2; MIP-1delta; Chemokine CC-2;Leukotactin-1;Mrp-2b; small inducible cytokine subfamily A (Cys-Cys), member 15.
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