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RNA isolation

  1. Remove the spleen from the sacrificed mouse according to the legal rules in your lab. Try to remove as much as possible of the remaining connective tissue but work quickly to avoid degradation of mRNA.
  2. Weight the spleen (~100-250 mg) and transfer it into a suitable container.
  3. Isolate the total RNA according to the protocol for the procedure/kit available to you.
  4. Dry the RNA pellet and dissolve in 100ul DEPC-treated water. Use immediately for RT-PCR

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