Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications. Highlights
Creative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsCompetitive binding assays, two (or more) ligands bind to the same receptor, are very important for biotherapeutic drug discovery. Relying on the state-of-art technology platform and profound expertise, Creative Biolabs offers a comprehensive range of competitive binding assay services to accelerate the customer's drug discovery process.
Ligand-receptor binding interactions are critical for biomedical research and drug discovery. Competitive binding assays are commonly used to measure the binding affinity of a ligand to its receptor. In this assay, the binding of a ligand labeled with a fluorescent or a radioactive tag is typically measured at a single concentration in the presence of different concentrations of an unlabeled, competing ligand. In order to measure binding affinity more accurately, a single binding protein is usually used in the assay. Either way, the competitive binding assay offers informative data to help guide your discovery program.
Creative Biolabs' immunologic analysis experts have abundant experience in developing a wide range of competition binding assay in a variety of formats, ensuring your project has the custom assay solution you need. We provide high-quality competition binding assay services, including but not limited to:
Radiolabelling binding assays are widely used to measure the binding of drugs to their targets (enzyme, receptor, etc.), especially when the native ligands are difficult to label by other means, such as fluorescence. This binding assay has many advantages, including high affinity, low non-specific binding, high specific activity to detect low receptor densities, and high receptor specificity. Using the competition binding format, we can determine the affinity of unlabeled compounds at equilibrium. Radiolabelling binding assays also enable us to explore the binding mechanism of drug molecules and determine the binding kinetics by performing time-course testing.
Fluorescence-based technologies are very important in pharmacological screening assays because they are amenable to miniaturization, and provide high sensitivity and signal-to-noise ratio.
1) Fluorescence polarization (FP)
FP is a robust and sensitive technique for measuring the binding between two molecules in solution. This method uses polarized light to excite the ligand and measures the amount of light emitted. Depolarization of the emitted light depends on ligand being bound (e.g., to the receptor). If the ligand is not bound, it will have a large depolarization (ligand is free to spin rapidly, rotating the light). If the ligand is bound, the combined larger size results in slower rotation and therefore reduces the degree of depolarization, which allows measurement of the interaction and the effect of small molecule inhibitors on this.
2) Fluorescence resonance energy transfer (FRET)
FRET is a valuable tool for studying molecular interactions within and between biomolecules and is being used more and more in biomedical research and drug discovery today. FRET relies on the distance-dependent transfer of energy from the donor molecule to the acceptor molecule. Like FP, FRET uses a fluorescently labeled ligand. Many FRET assays use variants of the green fluorescent protein (GFP) in both in vitro and in vivo applications. The dipole-dipole interaction between the donor and the acceptor molecule transfers the energy from the donor to the acceptor molecule. If the ligand binds to the receptor-antibody complex, then the acceptor will emit light.
Creative Biolabs's technical experts will help you choose the best service to meet your specific needs. For more details on how we can provide innovative support to your project, please feel free to contact us.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
