Creative Biolabs is one of the well-recognized experts who is professional in single domain antibody (sdAb) conjugation. Based on our world-leading antibody engineering platform, we can provide various sdAb-gold nanoparticle labeling services for our clients.
Due to the unique optical, electronic, and molecular-recognition properties, gold nanoparticles have been extensively used for applications both in biology and technology. Compared with other metal nanoparticles, gold nanoparticles have a high electron so that have been broadly used in electron microscopy. Furthermore, the stability and the capability to combine with biomolecules can be regarded as other prominent properties of such particles. The properties of gold nanoparticles, and thus their applications, depend strongly upon the shape and size. With these special characteristics, researchers attach molecules, such as protein and antibody, to gold nanoparticles that are attracted to diseased regions of the body, such as cancer tumors, and other molecules such as therapeutic drug molecules. This enables the functionalized gold nanoparticles to be used to in targeted drug delivery. Gold nanoparticles also have the capability to convert certain wavelengths of light into heat. Gold nanoparticles have been proved to be the most powerful tools in the nanomedicine application with the optical and electrochemical distinctiveness as well as the characteristics of biocompatibility.
Fig.1 Gold nanoparticle.
Single domain antibodies (sdAbs) are antibodies isolated from the serum of Camelidae and shark, or the specific developed human VH and VL domain whose size is about 1/10 of that of a conventional antibody. Unlike conventional antibodies, a single domain antibody consists only of heavy chains whose antigen-binding region is only a single domain linked to the Fc region through the hinge region. Due to the special structure, there are a series of advantages for the application of biological research. The single domain antibody fragments with decreased size, increased solubility, and higher stability have become of special interest for biotechnological and medical applications, including biosensors. Nowadays, single domain antibody conjugated gold nanoparticles have been widely applied to amplify the detection signal, cancer therapy and biosensor.
Above all, we are pleased to conjugate the gold nanoparticles with the target single domain antibody through two formats: (1). Direct attachment to the surface of the gold nanoparticle. (2). Covalent conjugation through a linker. On one hand, we can design the unique gold nanoparticles for achieving high efficiency and sensitivity in various research fields including bioanalysis, biomedicine, and nanotechnology, provide conjugation of single domain antibody to the gold nanoparticles with a wide range of available sizes. On the other hand, we can also help to purify and functionality test the conjugation production.
Based on the extensive experience of single domain antibody conjugation services and the advanced platform, Creative Biolabs is promising to offer the best service and the high-efficiency conjugation products to meet our clients’ demands precisely. In order to achieve the special goals for our clients, our technical scientists are pleased to communicate and decide the best experimental plan as soon as possible. If you are interested in our single domain antibody-gold nanoparticle conjugation service, please do not hesitate to inquire us for more details.