Introduction of SLC44A2
Solute carrier family 44 member 2 (SLC44A2), also known as choline transporter-like protein 2 (CTL2), is a transmembrane protein that in human is encoded by SLC44A1 gene. It is a member of the choline transporter-like (CTL) family and is functionally expressed as choline transporters. In humans, SLC44A2 mRNA and protein are found in the inner ear, trophoblastic cells, brain microvascular endothelial cells, glioblastoma cells, and tongue cancer cells. The intracellular localization of CTL2 is in the plasma membrane and mitochondria.
|Basic Information of SLC44A2|
|Protein Name||Choline transporter-like protein 2|
|Aliases||Solute carrier family 44 member 2|
|Organism||Homo sapiens (Human)|
Function of SLC44A2 Membrane Protein
The choline transport system has been assorted into three transporter families, including choline transporter-like proteins (CTLs), high-affinity choline transporter 1 (CHT1), and polyspecific organic cation transporters (OCTs). The CTL family consists of five members: CTL1, CTL2, CTL3, CTL4, CTL5. Recently, it has been proved that CTL2 exhibits detectable choline transport activity. However, the physiological function of CTL2 has not been fully characterized and the Km values for choline are still unknown. Choline is considered as a key substance for mitochondria since the deficiency of choline leads to mitochondrial and cellular oxidative injury as well as lipid peroxidation. CTL2 might play a role in choline uptake in mitochondria, which is the rate-limiting step in SAM (S-adenosyl-L-methionine) synthesis and DNA methylation. Thus, researchers hypothesize that CTL2 may be the predominant site for the control of choline oxidation in mitochondria and might be essential molecules of the apoptotic cell death in esophageal cancer cells. Furthermore, identification of the CTL2-mediated choline transport system may provide a potential novel target for esophageal cancer therapy.
Fig.1 Predicted structure of hCTL2. (Nair, 2004)
Application of SLC44A2 Membrane Protein in Literature
The results of this study show that the identification of the CTL2-mediated choline transport system may provide a potential novel target for esophageal cancer therapy.
This article suggests that choline is primarily transported by an intermediate-affinity choline transport system, CTL1 and CTL2, in hBMECs.
Authors of this study identify CTL-2 as a new binding partner for VWF (von Willebrand factor).
Authors of this study characterize reactions of HNA-3a antibodies, which can cause severe, sometimes fatal, transfusion-related acute lung injury when present in transfused blood, against recombinant versions of human, mouse, and human/mouse (chimeric) CTL2.
Results of this article prove that R154 in the context of full-length CTL2 is both necessary and sufficient to create the HNA-3a epitope, a target for antibodies that cause severe transfusion-related acute lung injury.
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