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SLC44A5 Membrane Protein Introduction

Introduction of SLC44A5

SLC44A5, also known as CTL5, is an integral membrane protein which belongs to the CTL (choline transporter-like) family. It is encoded by SLC44A5 gene and has 3 isoforms produced by alternative splicing. The longest isoform has 710 acid amino acid residues with 10 transmembrane helices, 6 cytoplasmic domains, and 5 extracellular domains. SLC44A5 is highly expressed in skin, testis, brain, colon, and esophagus.

Basic Information of SLC44A5
Protein Name Choline transporter-like protein 5
Gene Name SLC44A5
Aliases CTL5
Organism Homo sapiens (Human)
UniProt ID Q8NCS7
Transmembrane Times 10
Length (aa) 630
Sequence MNDTEKPADTPSEEEDFGDPRTYDPDFKGPVANRSCTDVLCCMIFLLCIIGYIVLGLVAWVHGDPRRAAYPTDSQGHFCGQKGTPNENKTILFYFNLLRCTSPSVLLNLQCPTTQICVSKCPEKFLTYVEMQLLYTKDKSYWEDYRQFCKTTAKPVKSLTQLLLDDDCPTAIFPSKPFLQRCFPDFSTKNGTLTIGSKMMFQDGNGGTRSVVELGIAANGINKLLDAKSLGLKVFEDYARTWYWILIGLTIAMVLSWIFLILLRFIAGCLFWVFMIGVIGIIGYGIWHCYQQYTNLQERPSSVLTIYDIGIQTNISMYFELQQTWFTFMIILCIIEVIVILMLIFLRNRIRVAIILLKEGSKAIGYVPSTLVYPALTFILLSICICYWVVTAVFLATSGVPVYKVIAPGGHCIHENQTCDPEIFNTTEIAKACPGALCNFAFYGGKSLYHQYIPTFHVYNLFVFLWLINFVIALGQCALAGAFATYYWAMKKPDDIPRYPLFTAFGRAIRYHTGSLAFGSLIIALIQMFKIVLEYLDHRLKRTQNTLSKFLQCCLRCCFWCLENAIKFLNRNAYIMIAIYGRNFCRSAKDAFNLLMRNVLKVAVTDEVTYFVLFLGKLLVAGSIGVLAFLFFTQRLPVIAQGPASLNYYWVPLLTVIFGSYLIAHGFFSVYAMCVETIFICFLEDLERNDGSTARPYFVTPNLHGILIKKQLVPQKQKE

Function of SLC44A5 Membrane Protein

SLC44A5 is a choline transporter which is involved in the membrane-spanning transport of choline. Although the functional research of SLC44A5 is relatively less, a study has shown that SLC44A5 is associated with hepatocellular carcinoma (HCC) carcinogenesis and progression. The higher expression levels of SLC44A5 are detected in the HCC tissues compared with the normal tissues. And SLC44A5 knockdown may downregulate the expression of cell cycle markers, cyclin-dependent kinase 2, and pro-proliferating cell nuclear antigen thereby suppressing cell viability and arresting the cells in the G1 phase of the cell cycle. Besides, SLC44A5 knockdown also reduces the expression levels of apoptosis markers, caspase-9 and caspase-3, resulting in cell apoptosis and invasion inhibition. Hence SLC44A5 can be regarded as a potential therapeutic target for HCC. In addition, cell viability assays suggest a role of SLC44A5 in the regulation of cell proliferation. And SLC44A5 knockdown promotes cell proliferation resulting in the larger body of calves which may lead to the rate of dystocia.

Biochemical network of ethanolamine and choline phospholipid metabolism showing crosstalk between these two metabolic cycles. (Cheng, 2016) Fig.1 Biochemical network of ethanolamine and choline phospholipid metabolism showing crosstalk between these two metabolic cycles. (Cheng, 2016)

Application of SLC44A5 Membrane Protein in Literature

  1. Peng G.Z., et al. Knockdown by shRNA identifies SLC44A5 as a potential therapeutic target in hepatocellular carcinoma. Molecular Medicine Reports. 2016, 13(6):4845-52. PubMed ID: 27082540

    The article demonstrates that SLC44A5 play a role in the hepatocellular carcinoma carcinogenesis and progression, indicating that SLC44A5 may be a potential therapeutic target for cancer.

  2. Sugimoto M., et al. The molecular effects of a polymorphism in the 5'UTR of solute carrier family 44, member 5 that is associated with birth weight in Holsteins. Plos One. 2012, 7(7):e41267. PubMed ID: 22815983

    The investigation suggests that reduced SLC44A5 expression may result in higher birth weights in calves via promoting cell proliferation.

  3. Lee K.T., et al. Neuronal genes for subcutaneous fat thickness in human and pig are identified by local genomic sequencing and combined SNP association study. Plos One. 2011, 6(2):e16356. PubMed ID: 21311593

    The study identifies eight neuronal genes (NEGR1, SLC44A5, PDE4B, LPHN2, ELTD1, ST6GALNAC3, ST6GALNAC5, and TTLL7) that are associated with subcutaneous fat thickness.

  4. Zhu Q., et al. Exomechip analyses identify genes affecting mortality after HLA-matched unrelated donor blood and marrow transplantation. Blood. 2018, 131(22): 2490-2499. PubMed ID: 29610366

    The study shows that SLC44A5 gene in donors is significantly associated with overall survival (OS) after HLA-matched unrelated donor blood and marrow transplantation.

SLC44A5 Preparation Options

Based on the versatile Magic™ membrane protein production platform, Creative Biolabs can help you to obtain the best soluble and functional target protein to make your project a success. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-SLC44A5 antibody development services.


As a forward-looking biotech company as well as a leading custom service provider in the field of membrane protein, Creative Biolabs has won good reputation among our worldwide customers for successfully accomplishing numerous challenging projects including generation of many functional membrane proteins. Our scientific team provides access to a comprehensive solution for membrane protein studies for our clients all over the world. Please feel free to contact us for more information.

Reference

  1. Cheng M, et al. (2016). Targeting phospholipid metabolism in cancer. Frontiers in Oncology. 6: 266.

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