Introduction of SLC46A1
SLC46A1, also known as proton-coupled folate transporter (PCFT), is an integral membrane protein which belongs to the CTL (choline transporter-like) family. It is encoded by SLC46A1 gene and has 459 acid amino acid residues. Topological analyses predict PCFT protein possesses 12 transmembrane helices, 7 cytoplasmic domains with the N-and C-termini location, and 6 extracellular domains. SLC46A1 is highly expressed in kidney, liver, placenta, small intestine, spleen, retina, and retinal pigment epithelium.
|Basic Information of SLC46A1|
|Protein Name||Proton-coupled folate transporter|
|Aliases||G21, HCP1, PCFT|
|Organism||Homo sapiens (Human)|
Function of SLC46A1 Membrane Protein
SLC46A1 is a transmembrane proton-coupled folate transporter protein that plays a role in folate transport across cell membranes, optimally in acidic pH environments. It is highly expressed in the apical membrane or cytoplasm of intestinal cells and mediates the folate absorption from the intestinal lumen. It is also expressed in the choroid plexus where it moves folates into the cerebrospinal fluid. Loss of PCFT function may lead to the autosomal recessive hereditary folate malabsorption disease, resulting in a syndrome characterized by severe systemic and cerebral folate deficiency. Moreover, SLC46A1 SNPs also have been associated with the risk of neural tube defects and oral clefts induced by folate deficiency. In addition, PCFT protein also acts as a heme transporter in duodenal enterocytes which regulates heme uptake from the gut lumen into duodenal epithelial cells. Mutations in mice SLC46A1 gene increase the levels of homocysteine and display severe hematopoietic deficits.
Fig.1 PCFT topology. (Zhao, 2017)
Application of SLC46A1 Membrane Protein in Literature
In this study, authors find nominally significant correlations between SLC46A1 SNPs and neural tube defects and oral clefts. But the results are still required to verify in other populations.
The article demonstrates the N411K mutant in the external gate of the proton-coupled folate transporter SLC46A1 is associated with hereditary folate malabsorption.
The study indicates a very important role of the 8th transmembrane helix of SLC46A1 in defining the aqueous channel and the folate binding pocket.
In this review, authors describe the functional and structural properties as well as regulation of SLC46A1 and contrast SLC46A1 properties with those of the reduced folate carrier (RFC).
The study implicates that the high expression of SLC46A1 and SLC19A1 are related to a decreased risk of recurrent colorectal cancer.
SLC46A1 Preparation Options
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