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HighlightsIntroduction of SLC4A2
SLC4A2, encoded by human SLC4A2 gene, also known as AE2, is a member of the SLC4 family of anion transporters which involves the bicarbonate transport across plasma membranes. The SLC4 polypeptides have several structural domains: two cytoplasmic domains, C- and N-terminal domains with 30-100 amino acids and 400-700 amino acids, respectively, and a C-terminal polytopic transmembrane domain with about 500 amino acids.
| Basic Information of SLC4A2 | |
| Protein Name | Anion exchange protein 2 |
| Gene Name | SLC4A2 |
| Aliases | AE2, HKB3, BND3L, NBND3, EPB3L1 |
| Organism | Homo sapiens (Human) |
| UniProt ID | P04920 |
| Transmembrane Times | 10 |
| Length (aa) | 1241 |
| Sequence | MSSAPRRPAKGADSFCTPEPESLGPGTPGFPEQEEDELHRTLGVERFEEILQEAGSRGGEEPGRSYGEEDFEYHRQSSHHIHHPLSTHLPPDARRRKTPQGPGRKPRRRPGASPTGETPTIEEGEEDEDEASEAEGARALTQPSPVSTPSSVQFFLQEDDSADRKAERTSPSSPAPLPHQEATPRASKGAQAGTQVEEAEAEAVAVASGTAGGDDGGASGRPLPKAQPGHRSYNLQERRRIGSMTGAEQALLPRVPTDEIEAQTLATADLDLMKSHRFEDVPGVRRHLVRKNAKGSTQSGREGREPGPTPRARPRAPHKPHEVFVELNELLLDKNQEPQWRETARWIKFEEDVEEETERWGKPHVASLSFRSLLELRRTLAHGAVLLDLDQQTLPGVAHQVVEQMVISDQIKAEDRANVLRALLLKHSHPSDEKDFSFPRNISAGSLGSLLGHHHGQGAESDPHVTEPLMGGVPETRLEVERERELPPPAPPAGITRSKSKHELKLLEKIPENAEATVVLVGCVEFLSRPTMAFVRLREAVELDAVLEVPVPVRFLFLLLGPSSANMDYHEIGRSISTLMSDKQFHEAAYLADEREDLLTAINAFLDCSVVLPPSEVQGEELLRSVAHFQRQMLKKREEQGRLLPTGAGLEPKSAQDKALLQMVEAAGAAEDDPLRRTGRPFGGLIRDVRRRYPHYLSDFRDALDPQCLAAVIFIYFAALSPAITFGGLLGEKTQDLIGVSELIMSTALQGVVFCLLGAQPLLVIGFSGPLLVFEEAFFSFCSSNHLEYLVGRVWIGFWLVFLALLMVALEGSFLVRFVSRFTQEIFAFLISLIFIYETFYKLVKIFQEHPLHGCSASNSSEVDGGENMTWAGARPTLGPGNRSLAGQSGQGKPRGQPNTALLSLVLMAGTFFIAFFLRKFKNSRFFPGRIRRVIGDFGVPIAILIMVLVDYSIEDTYTQKLSVPSGFSVTAPEKRGWVINPLGEKSPFPVWMMVASLLPAILVFILIFMETQITTLIISKKERMLQKGSGFHLDLLLIVAMGGICALFGLPWLAAATVRSVTHANALTVMSKAVAPGDKPKIQEVKEQRVTGLLVALLVGLSIVIGDLLRQIPLAVLFGIFLYMGVTSLNGIQFYERLHLLLMPPKHHPDVTYVKKVRTLRMHLFTALQLLCLALLWAVMSTAASLAFPFILILTVPLRMVVLTRIFTDREMKCLDANEAEPVFDEREGVDEYNEMPMPV |
Function of SLC4A2 Membrane Protein
SLC4A2 functions as Na+-independent electroneutral Cl--HCO3- exchanger that regulates electroneutral and reversible exchange of Cl- and HCO3- across the cell membrane. It is also involved in intracellular pH regulation and transepithelial acid-base transport. SLC4A2 has been shown to be expressed on the basolateral membranes of ileal and colonic epithelial cells in human where it is associated with vectorial chloride transport and maintenance of intracellular pH. Besides, SLC4A2 protein is also present in gastric parietal cells where it regulates acid secretion. The deficiency of SLC4A2 is involved in the pathogenesis of primary biliary cirrhosis (PBC). And mice with SLC4A2 knockdown have been shown as suitable animal models for studying PBC. The pathological study of PBC indicates that the decreased AE2 in lymphocytes may disorder pH regulation in these cells and change immune homeostasis resulting in autoimmunity, on the other hand, the reduced AE2 in cholangiocytes could lead to cholestasis and oxidative stress of bile duct cells.
Fig.1 Hepatobiliary transporters. (Joshi, 2010)
Application of SLC4A2 Membrane Protein in Literature
The findings show that the bile duct cells isolated from PBC patients exhibit defective Na+-independent Cl-/HCO3- exchange, indicating the role of AE2 deficiency in the pathogenesis of PBC.
The results of the present study reveal the potential role of spinophilin (SPL) and carbonic anhydrase XII (CA XII) in mediating AE2 activity, suggesting these regulatory proteins may act as the therapeutic targets for secretory diseases mediated by AE2.
In this research, the authors identify AE2 as a major pathway for basolateral Cl- loading during cAMP-stimulated secretion of Cl- and fluid by Calu-3 cells.
The article demonstrates a novel signal pathway involved in gastrin-stimulated AE2 expression mediated by EGR1 in gastric cancer cells.
Abscisic acid (ABA) is a hormone that regulates the responses to environmental stimuli, inflammatory responses, and glycemic concentrations. The research demonstrates ABA transport in human nucleated cells occurs via the anion exchanger AE2.
SLC4A2 Preparation Options
Based on the versatile Magic™ membrane protein production platform, Creative Biolabs can help you to obtain the best soluble and functional target protein to make your project a success. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-SLC4A2 antibody development services.
As a forward-looking biotech company as well as a leading custom service provider in the field of membrane protein, Creative Biolabs has won good reputation among our worldwide customers for successfully accomplishing numerous challenging projects including generation of many functional membrane proteins. Our scientific team provides access to a comprehensive solution for membrane protein studies for our clients all over the world. Please feel free to contact us for more information.
Reference
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