Introduction of SLC5A3
SLC5A3, also known as solute carrier family 5 (sodium/Myo-inositol cotransporter) member 3, solute carrier family 5 (inositol transporters) member 3, solute carrier family 5 (Inositol transporter) member 3, SMIT, SMIT1, or BCW2, is a transmembrane protein of 79.7 kDa that contains 718 amino acids. In humans, it is encoded by the SLC5A3 gene which resides on the chromosome 21q22.11. SLC5A3 belongs to the solute carrier family 5 (SLC5A) composed of 11 human members, all with 14 predicted transmembrane domains. SLC5A3 protein is responsible for the transport of sodium and Myo-inositol in response to hypertonic stress. SLC5A3 mRNA has been found in the brain, kidney, lung, pancreas, and placenta tissue, also in the heart and skeletal muscle.
|Basic Information of SLC5A3|
|Protein Name||Sodium/Myo-inositol cotransporter|
|Aliases||Na(+)/myo-inositol cotransporter, Sodium/myo-inositol transporter 1, SMIT1, Solute carrier family 5 member 3|
|Organism||Homo sapiens (Human)|
Function of SLC5A3 Membrane Protein
SLC5A3 is a plasma membrane protein in charge of the concentrative accumulation of Myo-inositol in numerous tissues. This protein cotransports sodium and Myo-inositol in a ratio of 2:1, and has the ability to transport many other sugars (e.g. scyllo-inositol, L-fucose, etc.) in Xenopus oocytes. In brain and renal cells, hypertonicity causes an increase in SLC5A3 expression thereby raising the cellular concentration of Myo-inositol. The renal medullary cells show high concentrations of Myo-inositol, betaine, sorbitol, and glycero-phosphocholine. Accumulation of these organic osmolytes helps cells to regulate their osmolarity in response to high extracellular osmolality which occurs in situations, such as concentrating urine. To date, there are other two Myo-inositol transporters have been identified, SLC5A11 (SMIT2) and HMIT. SLC5A11 has similar affinity for sodium and Myo-inositol as SLC5A3, but its activity differs in that it transports D-glucose and chiro-inositol, and cannot be inhibited by L-fructose.
Fig.1 Protein model for human SMIT1 and postulated splice variants. (Schneider, 2015)
Application of SLC5A3 Membrane Protein in Literature
This study proposed an integrative machine to analyze gene expression profiles in cervical cancer to identify a set of genetic markers. Functional analysis on 21 genes showed significant enrichment in a 9-potential gene expression signature, namely PEG3, SPON1, RPLP2 and BTD (upregulations) and SLC5A3, COPB2, PRDX3, LSM3 and AS1B (downregulations).
These results indicated that HMIT was expressed in basal cells and marginal cells of stria vascularis and SMIT1 expression was enriched in basal cells. The article speculated that HMIT and SMIT1 were likely to play essential roles in the homeostasis of cochlear fluids, for instance, by participating in pH regulation and osmoregulation.
Compared with TASK-1, three Na+-coupled nutrient transporters, SLC5A3, SLC5A6, and SLC38A1, were found to transport Myo-inositol, biotin, and glutamine, respectively, to be greatly overexpressed in lung adenocarcinomas. It was the first time that the TASK-1 channel modulated apoptosis and proliferation in a subset of non-small cell lung cancer.
This review demonstrated that the rs9982601 polymorphism of region between SLC5A3/MRPS6 and KCNE2 genes was correlated with long-term mortality in high-risk patients after myocardial infarction (MI). Furthermore, these findings also supported the previous reports on the relationship of the polymorphism and the prevalence of MI.
The article explored SLC5A3 protein as a potential transporter of Myo-inositol in hyponically swollen HEK293 cells and examined the correlation between the hypotonicity-induced outcomes in plasma membrane permeability to Myo-inositol Pino [m/s] and the expression of SLC5A3. These data first provided evidence for swelling-mediated activation of SLC5A3.
SLC5A3 Preparation Options
In obtain stable and fully functional membrane proteins of interest, a powerful Magic™ membrane protein production platform in Creative Biolabs would offer certain flexible options, from which you can always pick up the most suitable one to suit your needs. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-SLC5A3 antibody development services.
Creative Biolabs as a professional institute in protein manufacture fields has gained great reputation among worldwide followers for perfectly accomplishing varieties of challenging projects. Accordingly, we would like to recommend our systematic membrane protein preparation services to satisfy users’ requirements by using diverse strategies. Please feel free to contact us for more details.