The solute carrier (SLC) group of membrane transport proteins compromise over 400 members organized into 65 families. Solute carrier family 25, also known as adenine nucleotide translocator (ANT), is a class of transport proteins that exchange free ATP with free ADP across the inner mitochondrial membrane. ANTs are the most abundant proteins in the inner mitochondrial membrane, accounting for up to 10% of total mitochondrial protein content. ANTs play a central role in the transport of metabolites and cofactors across the mitochondrial inner membrane. They also mediate basal proton leak and mitochondrial permeability transition pore. ANT protein family has multiple isoforms in different species. In humans, ANTs have four isoforms (referred to as ANT1, ANT2, ANT3, and ANT4), which display distinct tissue-specific expression patterns.
ANTs have two subunits which are located in the inner mitochondrial membrane and contribute to the exchange of mitochondrial ATP and cytosolic ADP. They provide a continuous supply of ADP necessary to maintain the oxidative phosphorylation process. We're concerned here with several ANTs that you might be interested in.
Based on our Magic™ membrane protein production platform, scientists at Creative Biolabs have extensive experience in providing the preparation services of these targets in required formats using various strategies, including detergent micelles, proteoliposomes, nanodiscs, lipoparticles, polymers, with the stable cell line.
Moreover, our Magic™ membrane protein antibody discovery platform can also be applied in discovering antibodies against these targets, even fully humanized antibodies, by various approaches including hybridoma technology, phage display technology, and etc. We also present DNA immunization service for anti-membrane protein antibody development. Please feel free to contact us for more information.