Staphylococcal Nuclease Library Construction

Creative Biolabs provides the professional staphylococcal nuclease library construction service for customers all over the world. Our expert scientists are committed to offer services that are tailored to the individual demand of each client with the most appropriate project design.

Staphylococcal nuclease, also known as micrococcal nuclease, is an extracellular endo-exonuclease of Staphylococcus aureus that preferentially digests single-stranded nucleic acids. It is a small protein composed of 149 amino acid residues formed a no intrachain crosslinkages single peptide chain with molecular weight of 16.9kDa. The relevant 3D structure analysis of staphylococcal nuclease has indicated the molecule has three long α helices and a five-stranded, barrel-shaped β sheet which arranged as oligonucleotide-binding fold. Additionally, there is a prominently exposed loop on the surface of staphylococcal nuclease, which plays an important role in affinity contact with binders.

According to the compact structure and other featured characteristics, staphylococcal nuclease can be used as scaffold to develop specific binding ability. It is available to strongly express as a soluble protein in both eukaryotes and prokaryotes. In addition, the folding of staphylococcal nuclease protein is independent of the chaperones, meaning it has spontaneous fold ability. Thus, coupled with its small size and exposed surface loop, these properties have enabled staphylococcal nuclease to be used as scaffold or carrier protein in several significant studies in protein chemistry and as a practical tool in nucleic acid research. For instance, through inserting 16 random amino acids into the staphylococcal nuclease open reading frame (ORF) in place of the exposed loop, staphylococcal nuclease scaffold libraries were constructed and used to screen inhibitors of signal transduction pathways for the pathway identification.

Creative Biolabs take advantage of its proprietary Hi-Affi™ phage display platform to set up an efficient, stable and high affinity scaffold library construction. Base on the traditional phage display technology, which a gene of interest is typically fused to bacteriophage coat proteins and thereby displayed on the phage surface for selecting specific binders, our Hi-Affi™ platform also adopted trimer codon technology and NNK method to improve the library quality, which can obtain 100% precise mutant library construction with over 1010 diversity.

As an industry leader of the scaffold library construction, Creative Biolabs has always been providing the customers with the best quality service at most competitive price. Our experienced experts have generated more than 50 kinds of scaffold libraries and are confident in constructing the desired library for customers with any of their specific requirements.

Staphylococcal Nuclease Library Construction Fig. 1 Staphylococcal nuclease. (PDB ID: 2SNS)

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