Nervous System

Neurological disease is a rising burden for society and finance in aging societies, impacting approximately 1 billion individuals around the world, especially regarding neurodegenerative diseases including dementia, Alzheimer’s disease (AD), and Parkinson’s diseases (PD). The molecular basis of most neurodegenerative diseases is not fully understood till now, and the study of neurological disorders is particularly challenging. The emerging technology of iPSC attracts attention worldwide, giving access to genetic/epigenetic mechanism studies and new drug/methods exploration of neurodegenerative disease by generating patient-specific disease models in vitro. Creative Biolabs provides a series of patient-specific iPSC models of genetically inherited neurological diseases, which will satisfy different research demands for worldwide clients.

Together with gene editing techniques to reprogram human somatic cell into iPSC and differentiate iPSC into neurons, iPSC-based neurological disease models have revolutionized the utility of human in vitro models of neurological disease in the past decade, contributing to molecular/pathological mechanisms understanding, early diagnosis, drug discovery/screening as well as personalized precision medicine. Experienced ability is critical for generating iPSC-based models, including generating, expanding and purifying functional and disease-relevant neurological cells.

The Generation Steps of hPSC-based Disease Models

Determining an appropriate disease target or mutation
Directing hPSC differentiation into disease/phenotype-relevant cell populations
Identifying and purifying disease-relevant cells

Neurological Lineages Differentiation of hPSCs

Patients’ somatic cells like fibroblasts and other peripheral cells are collected and reprogramed into human pluripotent stem cells (hPSCs). Differentiation of hPSCs is regulated by particular cellular factors, mainly belonging to four signaling pathways (BMP, FGF, WNT/β-catenin and TGF-β/activin/nodal). Thus far, hPSCs have been successfully differentiated into cortical neurons, DA neurons, motor neurons, astrocyte, Purkinje cell, neural progenitor cells (NPCs) as well as neural stem cells (NSCs) by researchers around the world.

Fig 1. Directed Neural Differentiation of Human Pluripotent Stem Cells.

Features of Nervous System Disease Models

Rich experiences in iPSC reprogramming
A biologically relevant culture environment for iPSCs
Standardized operation protocol
Differentiated neurons highly relevant to disease phenotype
Customized services
Best after-sale services

hPSC Models of Neurological Diseases

hPSC-neurons (hPSC-NU) models of inherited neurological diseases are used to investigate neurological disease pathology mechanism and evaluate new medical methods and drugs. Creative Biolabs has established a state-of-the-art STEMOD^TM neurological disease models platform, to offer a list of hPSC-UN models with accurate evaluated neurological disease phenotypes. Those models are prepared for the investigation of Alzheimer’s disease, Parkinson’s disease and other inherited neurodegenerative disorders. Please feel free to contact us if you don’t find the proper neurological disease models for your research. Customized service will also be provided to meet every specific demand!

References

Chang, C.; et al. (2018). Induced Pluripotent Stem Cells. Cell Transplantation, 27(11), pp.1588-1602.
Park, I.; et al. (2008). Disease-Specific Induced Pluripotent Stem Cells. Cell, 134(5), pp.877-886.
Mungenast, A.; et al. (2016). Modeling Alzheimer's disease with human induced pluripotent stem (iPS) cells. Molecular and Cellular Neuroscience, 73, pp.13-31.

For Lab Research Use Only, Not for Human or Animal Therapeutic Use.

STEMOD™ AD Disease Model (PSEN1, A246E)(MoCNS-WQ001)