Overview Service Features FAQs Scientific Resources Related Services
As a world-leading service provider in the field of iPSC generation, Creative Biolabs offers various factor delivery systems for iPSC reprogramming. With years of research, we currently present a non-integrative approach for the factor delivery based on the Epstein Barr virus (EBV)-associated vector.
Introduction of Integration Free Vector System
As an alternative to integration-defective viruses, a novel iPSC reprogramming strategy based on integration-free vectors has been developed. This strategy can benefit from the simple implementation that can be achieved with a standard molecular biology experience, and avoid the labor-intensive and time-consuming viral particles production. A typical example is the OriP/Epstein–Barr nuclear antigen‑1‑based vectors (OriP/EBNA1) which contains two components: Orip and EBNA1. Once transfected into host cells, the EBNA1-encoded protein can be expressed via a viral promoter which enables recognize the Orip sequence and result in plasmid amplification following DNA amplification of the host cells. The vector removal from host cells can be achieved by culturing in the absence of drug selection. By co-transfecting several OriP/EBNA1 vectors that express different factor combinations, we have generated iPSCs from human foreskin fibroblasts (HFFs) successfully. It is noteworthy that the SV40LT antigen is used as one of the reprogramming factors because of the ability to inactivate both the retinoblastoma and the p53. Therefore, we suitably sacrifice reprogramming efficiency for better production safety against the potential tumorigenic risk.
Advantages Compared with Plasmid
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Higher transfection efficiency.
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Longer ectopic expression of the transgenes
Services at Creative Biolabs
With our well-established iPSC reprogramming factor delivery systems, the experienced scientists in Creative Biolabs are devoted to helping you with iPSC reprogramming and generation. We prefer to selecting the best delivery system according to your specific situation. Creative Biolabs also provides other services regarding iPSC technology, please do not hesitate to contact us for more details.
Here's an overview of how our service works.
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Key Processes
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Descriptions
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Cell Collection and Culturing
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We accept target cells from our customers and develop suitable conditions for culture.
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Cell Transfection
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The reprogramming factors are carefully chosen for their ability to induce pluripotency like OCT4, SOX2, KLF4, c-MYC, NANOG, LIN28.
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Our methodology involves a non-integrative system to transfect the cells. This method eliminates the need for viral vectors, reducing risks associated site mutations, immunogenic responses and biological waste. And our technology also presents a higher reprogramming efficiency.
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Colony Picking
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Once the cells have been transfected, they are cultured and monitored for the emergence of iPSC colonies. After approximately 14-21 days, iPSC colonies begin to appear.
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Cell Expansion
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We will expand for further characterization and validation. We ensure high-quality standards and validate every iPSC line by checking for pluripotency, trilineage differentiation potential, genomic stability and monitoring iPSC morphology.
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Deliverables
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Our clients will receive high-quality and integration-free iPSC lines, along with all the relevant QC data.
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Features of Our Services
Our service is a highly advanced and robust toolkit. This innovative and convenient service boasts several vital features, making it an attractive choice for researchers and businesses across the globe.
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Non-integrative System - Our vectors are non-integrative, ensuring your iPSCs don’t carry any unwanted, potentially mutagenic foreign DNA.
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Complete Service - From the initial isolation and culture of somatic cells, to the final validation of pluripotency, we provide a start-to-finish service.
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Safety and Removability - Integration-free vectors can be safely removed after the reprogramming process, leaving no residual traces in the iPSCs.
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Fast and Qualified - Our expert team provides a rapid turnaround, typically generating validated iPSC lines in less than two months. We maintain high-quality standards, providing full documentation for all reprogramming steps and validation results.
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High Efficiency - Our service utilizes an optimized process achieving high iPSC reprogramming efficiency, accelerating your research or product development pipeline.
Through these outstanding features, we provide a complete solution for iPSC reprogramming that we are confident will accelerate your stem cell research. Our service is a robust and reliable choice.
FAQs
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Q: Is there any risk or potential harm in this process to the cells?
A: Our method of reprogramming using integration-free vectors is one of the safest as it does not involve any genetic modifications or the use of viral vectors. However, like any biological process, there can potentially be some level of stress on the cells during reprogramming. We manage this through careful monitoring and optimized protocols to ensure the health and viability of the cells.
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Q: What types of cells can be reprogrammed using this service?
A: A wide range of human adult cells can be reprogrammed using our service, primarily blood cells and fibroblasts. However, the efficiency of reprogramming might vary among different cell types. Feel free to discuss your specific needs, and we'll advise on the best approach.
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Q: Can you ensure that the induced iPSCs will be free from the used vectors?
A: Yes, as part of our quality control, we perform multiple rounds of cellular passaging to ensure depletion of used vectors from the generated iPSCs, ensuring that you receive high purity, integration-free iPSCs.
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Q: Can I trust the efficiency of your method?
A: Absolutely. We utilize a robust reprogramming protocol that has been optimized for maximum efficiency. We have many satisfied customers who have successfully utilized our iPSCs for their advanced research studies.
Scientific Resources
For
Research Use Only. Not For Clinical Use.
