As a widely accepted and used tool in the field of mutagenesis and genomic modification, TALEN allowed rapid genome editing in a variety of cell types and different model organisms, having profound impacts on biological and medical research in recent years. With the advanced technology platform and experienced scientists, Creative Biolabs has employed TALEN services to generate innovative and effective cell therapy for our customers. Aided by our TALEN platform, you can revolutionize your particular project by enabling any site-specific genome editing without introducing foreign DNA.
Background of TALEN
TALEN (transcription-activator-like effector nuclease) technology has revolutionized the array of genome modifications in biotechnology and biomedical research. TALEN are restriction enzymes made by fusing a TAL effector DNA-binding domain to a DNA cleavage domain in a nuclease, which can create a site-specific double-stranded DNA break. Subsequently, the dsDNA break is repaired by cellular machinery and then creates a deletion mutation or a knock-in site for introducing site-specific DNA sequences of interest.
As effective molecular scissors, TALENs are widely used to induce a variety of specific and efficient genomic modifications in various cell types and different model organisms. With technology development, TALs can be engineered to deliver virtually any enzyme to any site and perform gene-editing functions, including gene knockout, knockin, mutagenesis, gene tagging, and for disease and cell therapeutics models. It is documented that TALENs have been used to engineer stably modified human embryonic stem cell and induced pluripotent stem cell (IPSCs), moreover, they have great promises in the cell therapy of cancers and genetic disorders, such as sickle cell disease and xeroderma pigmentosum.
Fig.1 Schematic diagram of the TALEN system. (Naitou, 2015)
TALEN Custom Services
Creative Biolabs offers complete custom TALEN products and services for genomic modification in a wide range of cell lines and organisms for our clients.
Donor Clones Design and Construction Service
Choosing an appropriate donor vector is an important first step of TALEN technology. Aided by our genome editing tools, Creative Biolabs provides customized plasmid vectors to specifically transfer your gene of interest. Moreover, our vectors can combine with different selection markers and genetic elements meeting your particular need. If your project requires this service, please prepare at least a 100 bp sequence of the region you would like the TALEN made against. Our donor clones possess the highest efficiency and minimal off-target effects.
Stable Cell Line Engineering Service
Stable cell lines can overcome the low transfection efficiency of transient expression, but it is time-consuming and complex to create stable cell lines. Creative Biolabs provides convenience by creating a variety of cell bank of monoclonal stable cell lines with TALEN-mediated genome modifications. From which, you can choose the best optimal candidate to help you quickly obtain the satisfactory results.
One-stop Service: You just need to tell us your purposed target and cell type, we will provide the high-performance stable cell line.
Wide Applicabilication: Our services can apply to targets of any genes and mammalian cells.
Guaranteed Quality: We will validate the efficiency of knock-out at mRNA or protein level upon request.
Transgenic Mice Services
Creative Biolabs can also offer transgenic mice with TALEN-mediated genome modifications for our clients. Our transgenic mice have been used within the scientific community around the world to study the gene functions and establish numerous animal models of human disease.
Fastest delivery of TAL effector plasmids or mRNA transcripts
Best after-sale service
Let's Work Together to Fulfil Your Genome Engineering Project
Creative Biolabs has successfully completed a lot of genome engineering projects aided by TALEN technology. It is exciting to offer our off-the-shelf product portfolio and outsourced services to help you get landmark development. But every project is different, if you don't find the TALEN services you need above, please contact us , we can customize our offering to meet your specific project needs.
Naitou, A.; et al. Heterodimeric TALENs induce targeted heritable mutations in the crustacean Daphnia magna. Biology open. 2015, 4(3): 364-369.