Single Cell Sequencing Service for Cancer

Single-cell sequencing technologies provide revolutionary tools for analyzing a single cell's gene expression, DNA variation, epigenetic state, and nuclear structure, thus improving the understanding of the molecular mechanisms for carcinogenesis and tumor progression. Creative Biolabs has accumulated rich experience in the field of single-cell sequencing. We provide the best and affordable single-cell sequencing services for cancer research based on two powerful technical platforms, 10X Genomics Chromium and BD Rhapsody.

Introduction of Single-Cell Sequencing

Single-cell sequencing allows the comprehensive and precise analysis of genome, transcriptome, and epigenome at single-cell resolution, which provides a better understanding of cellular heterogeneity and function in the context of its microenvironment. Especially, single-cell sequencing technologies provide powerful tools for tumor research, improving the understanding of intratumor heterogeneity, tumor microenvironment, metastasis, and therapeutic resistance. For example, carcinogenesis is a multistep and multifactorial process in which many types of tumor cells are formed, including primary tumor cells, metastatic tumor cells, cancer stem cells, circulating tumor cells, and disseminated tumor cells. Single-cell sequencing technologies can be used to investigate the cellular composition of primary tumor tissues and profile individual cancer cells from transcriptome, genome, and epigenome aspects, thereby deeply revealing the pathological mechanisms for tumors.

Table 1. Bulk vs. single-cell sequencing

	Single Cell Sequencing	Traditional Bulk Sequencing
Description	Amplifying and sequencing the DNA/RNA at a single cell level	Amplifying and sequencing the DNA/RNA at the level of a group of cells
Data Size	100-200Gb	6-10Gb
Application	Major in cancer biology, neurobiology, immunology, microbiology, embryology, tissue mosaicism, and so on	A range of biological research
Advantages	Accurately analyze genomic DNA, protein expression, and epigenetic signatures at the single-cell level; Examine the diversity of heterogeneous cell populations; Distinguish different cell types in a heterogeneous group of cells and identify a rare cell population Reveal the intercellular relationships in the microenvironment Cell lineage tracing study	Low cost Mature technology

Technologies

Creative Biolabs is equipped with two powerful single-cell sequencing platforms, 10X Genomics Chromium and BD Rhapsody, thus allowing capturing and analyzing thousands of cells per sample with low cost and high efficiency. The two platforms have been widely applied for multiple cancer research areas involving whole-genome analysis, transcriptome gene expression analysis, immune profiling, and epigenome analysis, at the level of single-cell, thus providing profound insights into tumor heterogeneity.10X Genomics Chromium system encapsulates 500 to 10,000 cells or nuclei per library together with micro-beads into nano-droplets. Each bead is loaded with oligonucleotides containing one of 750,000 different barcodes for the single-cell sequencing library preparation. BD Rhapsody is a nanowell-based system that allows high-throughput capture of nucleic acids from single cells using a simple cartridge workflow and a multitier barcoding system. The two platforms are compatible with several sequencers such as Illumina NovaSeq6000, HiSeq4000, NextSeq, and MiSeq.

Featured Services

Advantages

• Cutting-edge technologies to support high-quality single-cell sequencing services
• Accurate and reproducible data delivery
• Highly experienced expert team to provide real-time technical guidance
• Shorter experimental cycle to accelerate project success

If you are interested in our single-cell sequencing services, please feel free to contact us for more details.

1. Whole Genome Sequencing (WGS) Service for Cancer
2. Whole Exome Sequencing (WES) Service for Cancer
3. Targeted Sequencing Service for Cancer
4. Whole Transcriptome Sequencing (WTS) Service for Cancer
5. Immune Repertoire Sequencing (Rep-Seq) Service for Cancer
6. Epigenomics Service
7. Three-dimensional (3D) Genomics Service
8. Metagenomics Sequencing Service for Cancer
9. ctDNA Sequencing
10. Spatial Transcriptome Sequencing for Cancer