Creative Biolabs is an industry leader in the field of phage display technology. We can provide the best services of phage display library construction and phage display library screening. Our scientists have developed phage display platform to provide various excellent biopanning strategies for different purposes. The tailored biopanning strategy and proprietary protocol allow the increasing identification of synthetic polymers binding peptides with higher affinity and desired specificity. We are proud to use our extensive experience and advanced platform to offer this top-quality service for all of our global customers.
The Application of Synthetic Polymers in Biomedical Fields
The interfaces between synthetic polymers and binding peptides attract more and more attention to develop novel polymer materials utilized in biomedical ﬁelds, in particular, when proteins are readily adsorbed onto the hydrophobic or charged polymers surfaces. Therefore, the binding is extremely important in mediating various biological processes including cell adhesion and proliferation. However, it is normally difﬁcult to understand these interfacial phenomena, because the interfaces are composed of complicated interactions such as electrostatic, hydrogen bonding, or van der Waals forces. Researchers always employ vague terms such as "nonspeciﬁc" when proteins adsorb onto polymer surfaces. Phage display combinatorial libraries have been widely utilized to identify oligopeptides that speciﬁcally recognize the surfaces of artiﬁcial materials. The resulting peptides were utilized as novel functional nanomaterials. Next-generation sequencing (NGS) has emerged as a highly sensitive method for library analysis. NGS has been used to monitor from library quality control to hits discovery process, which makes it a powerful tool for better characterization of phage display libraries.
Fig. 1 Incorporation of the RGD motif to the PPyCl binding peptide identified by phage display enhanced cell adhesion on PPyCl substrates (Günay, and Klok 2015).
Based on our advanced phage display platform, Creative Biolabs has developed to provide various tailored biopanning strategies for the identification of synthetic polymers binding peptides with higher affinity and desired specificity.
Fig. 2 Representative synthetic polymers applied to the PD method. (a) poly(methyl methacrylate), (b) polylactide, (c) polystyrene, (d) poly(p-phenylene vinylene), (e) azobenzene-containing polymer, (f) polyetherimide and (g) poly(2-methoxy-5-propyloxysulfonate-1,4-phenylenevinylene) (Serizawa et al. 2011).
Synthetic Polymers as Substrates for Peptide Binder Identification
Abbreviated instructions: (PS) polystyrene; (PVC) poly(vinyl chloride); (α-PLLA) α-poly(L-lactide); (PMMA) poly(methyl methacrylate); (mpsPPV) poly(2-methoxy-5-propyloxysulfonate-1,4-phenylenevinylene); (PPV) poly(phenylene vinylene); (PDMS) poly(dimethylsiloxane); (PpyCl) chlorine-doped poly(pyrrole); (MA) methacrylate.
Applications of Synthetic Polymers Binding Peptides
In the field of applying phage display technology and high-throughput NGS technology, Creative Biolabs has accumulated extensive experiences. Based on our standard biopanning protocol, Creative Biolabs can tailor different strategies for synthetic polymers binding peptide identification to meet our clients’ project goals. For more detailed information, please feel free to contact us or directly send us an inquiry.