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TAC In Vitro Assays

Creative Biolabs is a leading global company that is dedicated to helping our worldwide customers shorten the discovery and development time in T Cell Antigen Connector (TAC) engineered T cell therapy. We offer a broad and integrated portfolio of laboratory and manufacturing services, especially for TAC engineered T cell design, construct, validation, and function assessment. Besides, we also have developed a systematic approach of TAC in vitro assays for TAC development, which should be crucial for your project. Our TAC in vitro assays including but not limited to:

T Cell Activation Assay

T cell, also known as T lymphocyte, is responsible for the initiation and regulation of the cell-mediated immune response. They can be divided into a variety of types based on their functions in immune response, including helper T cell, killer T cell, and regulatory T cell. T cell activation is an antigen-dependent process that leads to the proliferation and differentiation of T cells into effector cells. This process requires a primary activation signal to trigger intracellular signal transduction and new gene expression. Studies show that T cell activation plays an important role in immunotherapy and provides a novel method for T cell-associated disease therapy. We have established a novel method of T cell activation assay to match for your particular project, such as the detection of activation markers on the cell surface, the clonal expansion test of T cells, as well as TCR/CD3 effector cells detection assay.

Cytokine Release Assay

T cell engaging therapies have shown a promising result in the treatment of a wide variety of diseases, including solid and liquid tumors. However, the data also demonstrate that a series of adverse side effects have been found in patients, such as cytokine release syndrome (CRS). Therefore, we offer single or multiplex cytokine level analysis for your TAC-T cells using multiple animal models, including the immunodeficient mouse or rat model. Meanwhile, we also provide comprehensive cell content analysis for the assessment of non-tumor cell targeting of TAC-T therapy. Also, the expression levels of several memory markers, including CD45RA, CCR7, CD28, are detected for further identifying the function and the activation degree of TAC-T cells.

Cytotoxicity Assay

Studies indicate that engineered T cells have shown systemic and life-threatening adverse events in animal models or many clinical trials, including cytotoxicity, hypercytokinemia, and lysis syndrome. Therefore, in vitro cytotoxicity assays have been developed to evaluate the safety of engineered T cells. We provide a variety of efficient and innovative tests and reagents for the detection of cell viability and cytotoxicity. Various kits can be selected to detect cell viability or toxicity in a cell culture medium based on the luminescence reading. Meanwhile, many detection systems can be superimposed with apoptosis and other vitality detection reagents to determine the mechanism of cell death and to sensitively compare the data between holes, plates, and days. Besides, we also offer cytotoxicity analysis to determining effector: target ratio and cell necrotic risk assessments.

The Picture of TAC In Vitro Assays. - Creative BiolabsFig.1 The Picture of TAC In Vitro Assays.

Creative Biolabs offers a series of TAC in vitro assays which are part of our one-stop solution TAC engineered T cell therapy development services. With experienced teams of scientists, researchers, and technicians, we provide a fast turnaround, high-quality data at competitive prices for worldwide customers. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us for more details.

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