Creative Biolabs has over a decade of working experience in the field of phage display technology. Powered by our advanced phage display platform, we are dedicated to providing our customers with high-quality services of Phage Display Library Construction and Phage Display Library Screening. Based on our diverse custom and premade phage display antibody libraries, our seasoned scientists have developed a variety of strategies to identify thermal stable monoclonal antibodies (mAbs), including Fab, scFv, and VHH. We are pleased to share our cutting-edge technology and extensive expertise in identifying thermal stable mAbs to facilitate our clients’ research and project development.
Thermal Stable mAbs are Needed in Clinical Therapy
Antibodies and their derivative fragments have long been used as tools in a variety of applications, in fundamental research work, biotechnology, diagnosis, and even human therapy. Not surprisingly, the composition of antibodies in clinical trials is increasing. For most applications, besides the need of efficient biodistribution or reduced immunogenicity, thermals stability and solubility are critical factors. The beneﬁts of producing thermal stable mAbs are that they typically express at high levels in Escherichia coli and remain soluble in solution at higher concentrations for longer periods of time.
Thermal Stable mAbs Discovery in Creative Biolabs
In terms of the extensive experience in antibody research, Creative Biolabs has developed a variety of strategies for the identification of thermal stable mAbs. These strategies take advantage of the fact that bacteriophage M13 particles are heat stable. An antibody library is expressed on the surface of bacteriophage M13 when under high temperature, thermal unstable clones show reduced or no binding activity and will be lost during subsequent afﬁnity selection. However, thermal unstable clones that still retain binding to their target are selected and enriched with each subsequent round of afﬁnity selection. In order to conﬁrm that elevated temperatures are effective in eliminating thermal unstable phage clones, the phage library is heated at various temperature. Effect of high temperature on eliminating thermal unstable clones can be tested by phage ELISA. After the identification of thermal stable phage clones, the melting temperature (Tm) will be determined.
Fig.1 Regular antibodies (160 kDa) and their fragments (Doshi et al. 2014).
As an undisrupted global leader in antibody discovery and engineering, Creative Biolabs is proud to introduce our professional service of thermal stable mAbs discovery with our diverse strategies.
For more detailed information, please feel free to contact us or directly send us an inquiry.