Tyrosination Specific Antibody Discovery Service

Background Antibody Types Discovery Strategy Types of PTM Why Choose Us? FAQs

Creative Biolabs' Tyrosination-Specific Antibody Discovery Services deliver solutions for researchers challenged by accurate detection and study of protein tyrosination. Our use of advanced High-Affi™ technology enables us to deliver antibodies with exceptional specificity and reliability. Our organization provides complete solutions for both antibody discovery and production services.

Background

The post-translational modification of tyrosination adds a tyrosine residue to the C-terminal glutamate of α-tubulin. Microtubules depend on α-tubulin as a fundamental component, which serves essential structural roles in eukaryotic cells. The enzyme tubulin tyrosine ligase (TTL) drives this specific modification. Tyrosination and detyrosination both demonstrate evolutionary conservation while being essential to multiple cellular functions. The processes function to stabilize microtubules and mediate their interactions with microtubule-associated proteins (MAPs), while also determining the placement and activity of +TIPs (microtubule plus-end tracking proteins), which control both cell architecture and microtubule force distribution.

Fig. 1 Detyrosination/re-tyrosination cycle. (OA Literature). Fig.1 Schematic representation of the detyrosination/re-tyrosination cycle.¹

Research reveals that long-lasting microtubules contain detyrosinated tubulin while dynamic microtubules are composed of tyrosinated tubulin. Microtubule tyrosination plays a crucial role in maintaining normal growth and developmental processes. Several pathological conditions have been linked to abnormal tyrosination processes. The suppression of tyrosination has been documented in both animal and human cancer cases. Research into tyrosination dynamics and functions during normal physiological processes and disease states requires dependable tools like specific antibodies.

Antibody Types

Our Tyrosination-Specific Antibody Discovery Services enable you to access both monoclonal and polyclonal antibodies. Our antibody supply includes options derived from rabbit, mouse, and sheep host organisms. The antibody types include:

Site-Specific Anti-Tyrosination Antibodies: The antibodies have been engineered to detect and attach themselves to a precise location on the tyrosinated protein. The precise targeting and study of specific modifications depend on this antibody's high level of specificity.
Pan Anti-Tyrosination Antibodies: These antibodies detect tyrosination modification without respect to the protein's specific sites. Pan anti-tyrosination antibodies allow researchers to measure total tyrosination levels across proteins.

Discovery Strategy

Three main strategies guide our antibody development services.

Monospecific anti-tyrosination polyclonal antibody production: A designed immunogen composed of KLH-conjugated linear peptide that matches human tyrosinated α-tubulin typically serves to immunize host animals such as rabbits and sheep. Scientists refine the resulting polyclonal antibodies to enable precise targeting of the tyrosinated protein.

Fig. 2 General process of monospecific anti-tyrosination polyclonal antibody production. (Creative Biolabs Original)

Phage display strategy for anti-tyrosination monoclonal antibody discovery: This method produces monoclonal antibodies by generating a vast array of antibody fragments displayed on bacteriophage surfaces. The fragments undergo screening processes to identify those that bind to the tyrosinated protein, which enables researchers to isolate monoclonal antibodies that show high affinity and exquisite specificity for tyrosination.

Fig. 3 General process of phage display for anti-tyrosination monoclonal antibody discovery. (Creative Biolabs Original)

Hybridoma strategy for anti-tyrosination monoclonal antibody discovery: This technique involves immunizing a mouse with the target antigen before fusing its spleen B cells with myeloma cells to generate hybridomas. Hybridoma cells generate monoclonal antibodies that undergo screening processes to verify their specificity to tyrosination.

Fig. 4 General process of hybridoma for anti-tyrosination monoclonal antibody discovery. (Creative Biolabs Original)

Types of PTM

We provide tyrosination specific antibodies and antibodies targeting a variety of PTMs, as shown in the table below.

Why Choose Us?

Creative Biolabs stands as your leading partner for discovering antibodies that specifically target tyrosination. We provide:

High Specificity and Affinity: Through our High-Affi^TM technology, antibodies achieve exceptional selectivity toward tyrosination while reducing unwanted interaction with proteins lacking modifications.
Comprehensive Service Portfolio: We deliver end-to-end solutions, including immunogen design and antibody production through purification to final characterization.
Customized Solutions: We customize our services to address your research objectives and project specifications.
Expert Team: Our scientists, who specialize in antibody development, also demonstrate profound expertise in analyzing post-translational modifications.
Proven Track Record: Published research data confirms our antibody production services deliver reliable and effective results.

FAQs

Q: What methods ensure that the antibodies will target only tyrosination instead of other modifications?

A: The combination of High-Affi™ technology with our strict quality control methods delivers antibodies with minimal cross-reactivity to potential modifications. Highly specific antibodies result from our use of precisely developed immunogens together with advanced purification techniques. Our team can deliver precise information upon request.

Q: Which types of samples will work with your tyrosination-specific antibodies?

A: We can apply our antibodies across multiple techniques like Western blotting as well as immunofluorescence and immunoprecipitation and ELISA. Different antibodies may display varying compatibility with specific applications. Get in touch with our team so we can discuss your particular application needs.

Q: Which antibody type offers more benefits for my research application between monoclonal and polyclonal antibodies?

A: Monoclonal antibodies display consistent results across different batches and bind exclusively to one specific epitope. The ability of polyclonal antibodies to bind multiple epitopes enables enhanced detection signals. Your experimental requirements will determine which antibody option represents the optimal choice. Reach out to us to receive guidance on your decision.

Q: Do I need to follow specific guidelines when working with tyrosination-specific antibodies?

A: Following the recommended storage and handling instructions is essential for all antibodies. Your experiments require proper controls to achieve accurate and reliable outcomes. Reach out to us if you need detailed protocols.

Q: What sets your antibody services apart from those of competitors?

A: The distinction of Creative Biolabs lies in their dedication to high specificity, together with their extensive service offerings and tailored solutions. Our High-Affi™ technology and PTM antibody development capabilities work together to produce top-level results. For a detailed comparison, please reach out to us.

Creative Biolabs delivers top-notch Tyrosination Specific Antibody Discovery Services to speed up your research. Our service portfolio consists of specialized solutions designed according to your exact requirements. If you are interested in our services, please do not hesitate to contact us for more details.

Reference

Sanyal, Chadni, et al. "The detyrosination/re-tyrosination cycle of tubulin and its role and dysfunction in neurons and cardiomyocytes." Seminars in cell & developmental biology. Vol. 137. Academic Press, 2023. Distributed under Open Access license CC BY 4.0, without modification.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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