Filamentous Fungi Expression System for Vaccine Production

Vaccine production strategies mainly include live attenuated pathogens (viruses or bacteria) and inactivated vaccines based on killed microorganisms. In addition, there is a special vaccine, the subunit vaccine, which contains antigens isolated from pathogenic microorganisms. With the advent of recombinant DNA technology, it has become possible to prepare subunit vaccines independently of pathogens. Fungal expression systems, particularly yeast expression systems, and Filamentous Fungi Expression System, play an important role in the preparation of recombinant subunit vaccines. Creative Biolabs is a leader in the vaccine industry, with extensive experience in all phases and aspects from pre-design, pre-clinical evaluation to commercial production, especially in the expression of vaccines, with a variety of well-established expression systems and corresponding optimizations service, is your right-hand man in the vaccine development process.

Filamentous fungi are lower eukaryotes commonly used to produce subunit vaccines with outstanding advantages including excellent protein secretion and post-translational modification capabilities similar to other eukaryotic proteins. The secreted proteins produced by filamentous fungi undergo a process of folding, proteolytic processing and other modifications such as glycosylation, giving them a similar function as natural proteins. Mainly applied species for vaccine production are Aspergillus niger, A. awamori, A. oryzae, and Trichoderma reesei. The productivity of A. niger is up to 25-30 g/ liter in terms of secretory recombinant protein and the more productive T. reesei is able to produce protein in a yield of 100 g/ liter in controlled fermentative.

Fig. 2 Comparison of several expressing system. (Nevalainen H and Peterson R, 2018)

Filamentous fungi have relatively lower gene transformation efficiency than other expression systems. Common transformation methods are PEG-mediated transformation, biolistic, electroporation of fungal chloroplasts, Agrobacterium tumefaciens–mediated transformation and the latest underwater shock waves, among the numerous measures, PEG-mediated transformation is widely used due to its easy manipulation.

Strategies to Cultivate a Stable Filamentous Fungi Strain

• Manipulate genomes of the host to remove harmful proteases
• Optimize pH of the culture medium
• Adjust concentrations and types of carbon and nitrogen sources

Platforms

• Multiple and well-established genetic engineered filamentous fungi expressing strains
• A series of alternative promoters that meet different requirements
• Manifold selectable markers
• One-stop vaccine manufacturing service

Creative Biolabs is a professional vaccine development and service company with decades of experience in the field of vaccines. Our team of outstanding scientists from around the world, focusing on vaccine research, aims to provide you with the best strategies and services.

Reference

1. Nevalainen H (2018). “Overview of Gene Expression Using Filamentous Fungi” Curr Protoc Protein Sci. 2018 Apr;92(1)

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