Recombinant Human FLT3 Protein (aa 1-541) [His] (VAng-Cr4000)

Recombinant Human FLT3 Protein (Met 1-Asn 541) fused with a His tag at C-terminus was expressed in HEK293 Cells, with a molecular weight of 59.7 kDa. (Accession ID: NP_004110.2)

Datasheet

Specifications

Source

Human

Target

FLT3

Type

Tumor Antigens

Applications

ELISA.

Formulation

Lyophilized from sterile PBS, pH 7.4.

Purity

> 85% pure by SDS-PAGE.

Storage

Store at -20°C to -80°C.

Antigen Information

Introduction

FLT3 is a cytokine receptor which belongs to the receptor tyrosine kinase class III. CD135 is the receptor for the cytokine Flt3 ligand (FLT3L). It is expressed on the surface of many hematopoietic progenitor cells. Signalling of FLT3 is important for the normal development of haematopoietic stem cells and progenitor cells. FLT3 is composed of five extracellular immunoglobulin-like domains, an extracellular domain, a transmembrane domain, a juxtamembrane domain and a tyrosine-kinase domain consisting of 2 lobes that are connected by a tyrosine-kinase insert. Cytoplasmic FLT3 undergoes glycosylation, which promotes localization of the receptor to the membrane. When this receptor binds to FLT3L a ternary complex is formed in which two FLT3 molecules are bridged by one (homodimeric) FLT3L. The formation of such complex brings the two intracellular domains in close proximity to each other, eliciting initial trans-phosphorylation of each kinase domain. This initial phosphorylation event further activates the intrinsic tyrosine kinase activity, which in turn phosphorylates and activates signal transduction molecules that propagate the signal in the cell. Signaling through CD135 plays a role in cell survival, proliferation, and differentiation.

Alternative Names

CD135; FLK-2; FLK2; STK1; fms related tyrosine kinase 3