Recombinant Human IGFBP-3 Protein (aa 28-291) [His] (VAng-Cr4015)

Recombinant Human IGFBP-3 Protein (Gly28-Lys 291) fused with a His tag at N-terminus was expressed in HEK293 Cells, with a molecular weight of 31 kDa. (Accession ID: NP_000589.2)

Datasheet

Specifications

Source

Human

Target

IGFBP-3

Type

Tumor Antigens

Applications

ELISA.

Formulation

Lyophilized from sterile PBS, pH 7.4.

Purity

> 85% pure by SDS-PAGE.

Storage

Store at -20°C to -80°C.

Antigen Information

Introduction

IGFBP-3 is one of six IGF binding proteins (IGFBP-1 to IGFBP-6) that have highly conserved structures and bind the insulin-like growth factors IGF-1 and IGF-2 with high affinity. IGFBP-7, sometimes inappropriately included in this family, shares neither the conserved structural features nor the high IGF affinity. It has well-documented functions in the circulation, in the extracellular environment, and inside cells. It is the main IGF transport protein in the bloodstream, where it carries the growth factors predominantly in stable complexes that contain the binding protein, either IGF-1 or IGF-2, and a third protein called the acid-labile subunit or ALS. For IGFs to reach the tissues from the bloodstream, the circulating complexes are believed to partly dissociate, possibly enhanced by limited proteolysis of IGFBP-3. The IGF-1/IGFBP-3 ratio has sometimes been used as an index of IGF bioavailability in the human circulation, but this ignores IGF-1 binding to other IGFBPs (so the ratio is affected by the concentrations of all six IGFBPs), and the fact that IGF-2, which is three times more abundant than IGF-1 in the bloodstream of adults, occupies the majority of binding sites on circulating IGFBP-3.

Alternative Names

BP-53; IBP3; insulin like growth factor binding protein 3