Recombinant YFV NS4A Protein (aa 1-3411) (strain Ghana/Asibi/1927) (VAng-Cr6547)

E. coli or Yeast or Baculovirus or Mammalian Cell.

Yellow Fever Virus

Viral Antigens

ELISA, WB, SDS-PAGE, Immunogen, etc.

Tris-based buffer, 50% glycerol.

> 85% pure by SDS-PAGE.

Store at -20°C to -80°C.

Yellow fever virus is a 40– to 50-nm-wide enveloped RNA virus and is mainly transmitted through the bite of the yellow fever mosquito. The positive-sense, single-stranded RNA is around 11,000 nucleotides long and has a single open reading frame encoding a polyprotein. Host proteases cut this polyprotein into three structural (C, prM, E) and seven nonstructural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, NS5); the enumeration corresponds to the arrangement of the protein coding genes in the genome. The yellow fever virus is taken up by a female mosquito when it ingests the blood of an infected human or another primate. Viruses reach the stomach of the mosquito, and if the virus concentration is high enough, the virions can infect epithelial cells and replicate there. From there, they reach the haemocoel (the blood system of mosquitoes) and from there the salivary glands. When the mosquito next sucks blood, it injects its saliva into the wound, and the virus reaches the bloodstream of the bitten person.

YFV