What is the core difference between subunit vaccines and traditional vaccines?

Subunit vaccines contain only specific antigens like proteins or peptides to stimulate an immune response, unlike traditional vaccines that may use live-attenuated or inactivated whole microbes, offering higher safety by excluding non-essential microbial components.

What are the key advantages of subunit vaccines?

They offer safety with reduced risks of adverse reactions due to the absence of non-antigenic microbial elements, suit individuals with weakened immune systems, and can induce long-lived immunity through proper formulation.

What are the main challenges in subunit vaccine development?

Challenges include low immunogenicity requiring adjuvants to enhance the immune response, the need for multiple doses to achieve long-term immunity, and the complex and time-consuming process of identifying the most effective antigens.

Can you provide examples of recombinant subunit vaccines?

Examples include the Hepatitis B vaccine (producing HBsAg in yeast), the human papillomavirus (HPV) vaccine, and some influenza vaccines, all using purified recombinant proteins to trigger immune responses.

Which antigens are commonly used?

Examples include viral surface proteins (e.g., SARS-CoV-2 Spike), bacterial toxins (e.g., diphtheria toxoid), and polysaccharide conjugates (e.g., Hib vaccine).

What services does Creative Biolabs offer for subunit vaccine design?

Creative Biolabs provides custom antigen selection and design, recombinant subunit vaccine development using prokaryotic (e.g., E. coli) and eukaryotic (e.g., yeast, mammalian cells) expression systems, expertise in adjuvant selection and formulation, and preclinical evaluation support through advanced platforms and decades of vaccine technology experience, with all products for research use only.

Subunit Vaccine Solution

At Creative Biolabs, we understand the critical need for safe and effective subunit vaccines. While many providers offer general vaccine development, our subunit vaccine Solutions stand out by focusing on comprehensive antigen identification, optimized expression systems, and advanced adjuvant screening. We leverage cutting-edge technologies to deliver highly purified and immunogenic protein or peptide antigens, ensuring minimal reactogenicity and robust immune responses.

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End-to-end subunit vaccine solutions

What is subunit vaccine?

Subunit vaccine is a type of vaccine that presents antigens which best stimulate the immune response to the immune system, instead of the entire microbe. One production method involves isolating specific proteins from the virus and administering them on their own. Subunit vaccines contain only the necessary antigens, not all other molecules that make up the microbes, so the likelihood of adverse reactions to the vaccine is very low. However, they have low immunogenicity and need to be used with good adjuvant, and multiple doses are needed for long-term immunity.

Comprehensive Subunit Vaccines Preclinical Solutions

At Creative Biolabs, we've long been dedicated to vaccine technology development. We offer subunit vaccine design services. We can produce subunit vaccines by isolating specific proteins from viruses and administering them. Also, we use recombinant DNA tech to create antigen molecules. With our years of experience and advanced platforms, we can provide customized vaccine design services based on your needs.

Comprehensive Service Portfolio

Vaccine Design Adjuvant selection Analysis & Validation

Customized Subunit Vaccine Design

Antigen Identification: Customized subunit vaccines for bacterial (e.g., Streptococcus, E. coli), viral (e.g., HIV, SARS-CoV-2), and parasitic pathogens, focusing on key antigens like toxins, surface proteins, and adhesion factors.
Multi-Epitope Engineering: Advanced bioinformatics-driven design of multi-epitope vaccines to enhance immunogenicity and cross-protection against diverse pathogen strains.
Technology Integration: We leverage recombinant protein expression and bacterial vector systems for efficient antigen delivery.

Adjuvant Development & Optimization

Comprehensive Adjuvant Screening: Evaluation of diverse adjuvants (e.g., aluminum salts, oil-in-water emulsions, TLR agonists) to enhance immunogenicity for subunit, DNA, and viral vector vaccines.
Mechanistic Profiling: In-depth in vitro/in vivo studies to assess adjuvant effects on dendritic cell activation, cytokine induction, and long-term immune memory.
Novel Adjuvant Design: Development of next-gen adjuvants (e.g., STING agonists, saponin-based platforms) for challenging pathogens or immuno-resistant targets.

Analytical Development & Preclinical Validation

Quality Control Assays: Comprehensive antigen characterization (HPLC, SDS-PAGE), potency testing, and residual impurity detection (host cell proteins, endotoxins).
Immunogenicity Profiling: Neutralizing antibody assays (PRNT, MN), T-cell response analysis (ELISpot, intracellular staining), and functional efficacy studies.
Preclinical Safety: Toxicity testing (single/repeat dose), local tolerance assessment, and histopathological evaluation.

Featured Subunit Vaccine Design Services

Structure based Vaccine Design

Structural Guidance: Uses antigen structural information for rational design and targeted immunity.
Epitope Optimization: Enhances immunogenicity and efficacy through structural epitope analysis.
Recombinant Production: Employs recombinant technology and diverse expression systems for defined components.

Structure based Immunogen Design

Structural Engineering: Uses structural biology for optimal epitope exposure and robust immunity.
Native Mimicry: Considers PTMs & conformations to mimic native antigens.
Computational Refinement: Employs computational modeling to predict interactions and refine designs.

How Dose Subunit Vaccine Solution Work?

1. Antigen Design and Optimization

High-throughput sequencing for pathogen genome analysis
AI-predicted immunogenic epitopes
Polysaccharide-protein conjugate design (e.g., S. pneumoniae CPS)

2. Immunization Strategy Development

Outer Membrane Vesicle (OMV) platform for antigen expression
Adjuvant optimization (aluminum salts, TLR agonists)
Customized delivery systems (nanoparticles/liposomes)

3. Immunogenicity Assessment

Humoral immunity: IgG/IgA subtypes, neutralizing antibodies
Cellular immunity: Th1/Th2/Th17 response profiling
ADCP/ADCD functional validation

4. Animal Model Validation

Species-specific challenge models (mouse/guinea pig/rabbit)
Efficacy evaluation (LD50, bacterial load)
Safety assessment (local/systemic toxicity)

5. Reporting

Antigen screening reports
Test data compilation
Project design documentation

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Getting Started with Your Subunit Vaccine Project

Professional Vaccine Solution platform

Antigen Prediction System Platform

Our Antigen Prediction System Platform integrates advanced bioinformatics and computational biology tools to identify and prioritize immunogenic antigens. Leveraging cutting-edge algorithms, we analyze pathogen genomes or cancer markers to predict key epitopes that elicit robust immune responses. This platform enables the rational design of vaccines by pinpointing high-potency antigens, significantly accelerating the development process for bacterial, viral, fungal, parasitic, and cancer-targeted vaccines. By combining traditional immunology with state-of-the-art predictive modeling, we ensure optimal antigen selection for maximum immunogenicity and efficacy.

Protein Expression Platform

The Protein Expression Platform at Creative Biolabs offers versatile solutions for producing recombinant antigens using multiple expression systems. We specialize in optimizing bacterial (e.g., E. coli), yeast, insect, and mammalian cell systems to meet diverse production needs. Our expertise in genetic engineering allows us to tailor expression vectors, culture conditions, and purification protocols for high-yield, biologically active proteins. Whether for preclinical research or large-scale manufacturing, our platform ensures consistent production of immunogenic proteins, supporting the development of vaccines with enhanced stability and immunogenicity. We seamlessly integrate expression technologies with downstream analytical and formulation services to streamline the vaccine development pipeline.

Modular Delivery System Platform

Our Modular Delivery System Platform is designed to enhance vaccine potency and delivery efficiency through customizable, multi-component formulations. Focused on optimizing delivery mechanisms, we develop lipid-based nanoparticles, viral-like particles (VLPs), and other nano-carriers to protect antigens, improve cellular uptake, and modulate immune responses. The modular design allows for precise integration of adjuvants, targeting ligands, and protective matrices, enabling tailor-made systems for various vaccine types—from prophylactic to therapeutic.

Why Choose our Bacterial Vaccines Services?

Structure-Based Antigen Design

We engineer high-purity antigens using precise structural modeling of target epitopes, enabling self-assembled trimeric nanoparticles for enhanced immunogenicity and cross-neutralization against variants.

Nano-Delivery & Adjuvant Systems

Our proprietary nanoparticle platforms mimic pathogen structures, boosting dendritic cell uptake and lymph-node targeting to elevate T-cell responses and antibody titers beyond conventional approaches.

Rapid Response to Emerging Threats

Leveraging high-efficiency expression platforms, we accelerate vector construction and GMP-grade antigen production—critical for timely deployment against evolving pathogens like XBB lineages.

Tailored Novel Adjuvant Integration

We integrate customizable adjuvants to overcome subunit vaccine limitations, significantly amplifying neutralizing antibody breadth and durability even in single-dose regimens.

Available Products

Antigen Products

Creative Biolabs offers ready-to-use bacterial or viral antigens to accelerate research timelines, while also providing customized antigen solutions tailored to specific experimental needs upon request

Viral Antigen Products

Hepatitis B Virus Human Papillomavirus Coronavirus Norovirus Monkeypox Virus Influenza A Virus

Bacterial Antigen Products

Streptococcus Pneumoniae Corynebacterium Diphtheriae Haemophilus Influenzae Vibrio Cholerae

Antibody Products

Creative Biolabs delivers off-the-shelf bacterial or viral antibodies to streamline research workflows, with custom antibody design services available for specialized applications. Find more antibodies>>>

CAT	Product Name	Price
VAnt-Wyb650	NoV Monoclonal Antibody (IgG2a)	-
VACY-0922-CY143	Anti-Monkeypox (MPXV) M1R Monoclonal Antibody	$1380.00
VACY-0922-CY142	Anti-Monkeypox (MPXV) E8L Monoclonal Antibody	$1380.00
VACY-0922-CY141	Anti-Monkeypox (MPXV) A35R Monoclonal Antibody	$1380.00
VACY-0922-CY140	Anti-Monkeypox (MPXV) A29L Monoclonal Antibody	$1380.00
VASX-0522-SX4	Anti-Monkeypox Virus Monoclonal Antibody (VACV-5C7), Mouse IgG1, Kappa	$1380.00

FAQs About Our Subunit Vaccine Solution

What is the core difference between subunit vaccines and traditional vaccines?

Subunit vaccines contain only specific antigens like proteins or peptides to stimulate an immune response, unlike traditional vaccines that may use live-attenuated or inactivated whole microbes, offering higher safety by excluding non-essential microbial components.
What are the key advantages of subunit vaccines?

They offer safety with reduced risks of adverse reactions due to the absence of non-antigenic microbial elements, suit individuals with weakened immune systems, and can induce long-lived immunity through proper formulation.
What are the main challenges in subunit vaccine development?

Challenges include low immunogenicity requiring adjuvants to enhance the immune response, the need for multiple doses to achieve long-term immunity, and the complex and time-consuming process of identifying the most effective antigens.
Can you provide examples of recombinant subunit vaccines?

Examples include the Hepatitis B vaccine (producing HBsAg in yeast), the human papillomavirus (HPV) vaccine, and some influenza vaccines, all using purified recombinant proteins to trigger immune responses.
Which antigens are commonly used?

Examples include viral surface proteins (e.g., SARS-CoV-2 Spike), bacterial toxins (e.g., diphtheria toxoid), and polysaccharide conjugates (e.g., Hib vaccine).
What services does Creative Biolabs offer for subunit vaccine design?

Creative Biolabs provides custom antigen selection and design, recombinant subunit vaccine development using prokaryotic (e.g., E. coli) and eukaryotic (e.g., yeast, mammalian cells) expression systems, expertise in adjuvant selection and formulation, and preclinical evaluation support through advanced platforms and decades of vaccine technology experience, with all products for research use only.