Insect Cell based Virus-Like Particle (VLP) Production Service

Creative Biolabs is a well-recognized pioneer in offering all-around virus-like particle (VLP) services. We have developed a series of advanced systems for VLP construction. In particular, we now offer best-in-class VLP production service based on our unparalleled insect expression system. We strive to identify the most critical needs of global customers, and we keep challenging ourselves to meet their needs through continuous innovation.

Introduction of VLP Production in Insect Cell Expression System

One or more viral structural capsid proteins spontaneously interact to generate the final structure of VLPs. While VLPs lack either the entire virus genome or a complete virus genome, they resemble live viruses physically and aesthetically. VLPs can be produced on a variety of expression platforms, such as eukaryotic and prokaryotic systems. The most widely used expression system for producing enveloped and non-enveloped VLPs is baculovirus/insect cells. The expression system can benefit from a number of factors, including the high yield of produced proteins from insect cell expression systems, which is comparable to that of proteins obtained from bacteria or yeast, the presence of complex PTM pathways, and the formation of multi-protein VLPs.

VLPs production in the insect expression system (Sedova, et al. 2012) Fig.1 Production of VLPs in the baculovirus-insect expression system (A: recombinant baculovirus; B: transfection of insect cells; C: VLPs).¹

Workflow of VLP Production in Insect Cell Expression System

We were able to express and assemble the virus structure proteins to generate VLPs that were identical to the virions. The goal of Creative Biolabs was to produce and characterize a particle similar to the VLPs and express its structural proteins utilizing a baculovirus-insect expression system.

Step	Details
Gene Synthesis and Cloning	Confirm the gene of interest Gene synthesis and cloning into baculovirus-insect expression vector
Preparation of Recombinant Baculovirus	Transform into DH10Bac cells to generate bacmid DNAs. Validate bacmid DNA by PCR analysis
Transfection Insect Cells	Transfect bacmid DNA into insect cells
Assembly of VLPs	Validate the protein expression and assembly by western blot or SDS-PAGE
Purification of VLPs	Purify VLP from either cultured medium or cell lysate using the combination method of gradient centrifugation and chromatography.

Creative Biolabs is pleased to tailor the most appropriate experimental scheme for our customers based on different research directions. Alternative expression systems are also available from which we can select the most appropriate choice to proceed:

VLP Production in E. coli System
VLP Production in Yeast Cell System
VLP Production in Plant System
VLP Production in Mammalian Cell System

We are committed to being the premier, research-intensive biotechnology company and are dedicated to providing leading solutions for old and new customers. We are always dedicated to the highest level of scientific excellence. Please feel free to contact us for further discussions.

Reference

Sedova, E. S., et al. "Recombinant influenza vaccines." Acta Naturae (англоязычная версия) 4.4 (15) (2012): 17-27. Distributed under Open Access license CC BY 4.0, without modification.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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