Mammalian Cell based Virus-Like Particle (VLP) Production Service

Creative Biolabs is an undisputed world-leading service provider of virus-like particles (VLPs) technology. With rich experience and well-established platforms, we can offer leading-edge virus-like particles (VLPs) production service based on our advanced mammalian cells system to deliver diverse research goals. Our professional experts are well-adept and pleased to tailor the most reliable and cost-effective strategies for our customers to facilitate their significant research.

Introduction of VLP Production in Mammalian Cell Expression System

VLPs are non-infectious multiprotein complexes that do not carry the viral DNA that causes disease. The most useful and appealing platforms for creating numerous structural proteins of both enveloped and non-enveloped VLPs are mammalian cell expression systems. Because animal cell expression platforms can produce precise and complicated PTMs, which are necessary for correct protein folding, they are the most effective methods for producing recombinant proteins. The CAP-T cell line has also been demonstrated to be a highly effective expression system for HIV VLP generation. VLPs against the influenza, rabies, and HIV viruses have been produced using the HEK293 cell line. However, it is thought that the main drawbacks of using mammalian cell expression systems to produce material for clinical use are their low protein output, high production costs, lengthy-expression times, and propensity for cell lines to harbor infections from mammalian pathogens.

VLPs production in the mammalian cell expression system. (Nooraei, et al. 2021) Fig.1 Production of VLPs in the mammalian cell expression system¹

Workflow of VLP Production in Mammalian Cell Expression System

Among all these systems available, statistical data showed that more than half of all recombinant proteins in the pharmaceutical industry are produced in mammalian cell systems. VLPs can serve as a novel class of nanoparticles, especially to present structural proteins as immunogens, bypassing the need for live pathogen or recombinant viral vector preparation.

Step	Details
Gene Synthesis and Cloning	Confirm the gene of interest Gene synthesis and cloning into mammalian expression vector
Transfection Mammalian Cells	Transfect expression plasmids into mammalian cells
Assembly of VLPs	Validate the protein expression and assembly by western blot or SDS-PAGE
Purification of VLPs	Purify VLP from either cultured medium or cell lysate using the combination method of gradient centrifugation and chromatography.

Creative Biolabs offers one-stop VLP construction services for diverse research/industrial needs. We promise our clients first-class service from initial inquiry to post-delivery with a Ph.D. technical support staff assigned to your project. High-efficient and excellent representing services have earned us the trust from a great deal of customers all over the world. Moreover, many well-qualified VLP products can also be provided as customized products. Please feel free to contact us for further discussions.

Reference

Nooraei, Saghi, et al. "Virus-like particles: preparation, immunogenicity and their roles as nanovaccines and drug nanocarriers." Journal of Nanobiotechnology 19 (2021): 1-27. Distributed under Open Access license CC BY 4.0, without modification.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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