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Virus-Like Particles (VLPs) Production in Plant System

Creative Biolabs is a global-leading biotechnology company devoting to the first-class VLP-related services. Using our innovative technology, we have extensive experience in assembling a variety of VLP products derived from various virus families including HBV VLP, HIV VLP, HPV VLP, CA16 VLP, EV71 VLP, poliovirus VLP, etc. As one trustworthy scientific partner, we are always pleased to leverage our professional skills to help customers with their diverse research and projects. In particular, we have established an innovative plant-based system for VLP manufacture.

Viruses like particles (VLPs), assembled from capsid protein subunits of one or several different viruses, have been applied in a wide range of fields such as vaccines, antibody development, membrane protein expression, delivery systems, bioimaging and cell targeting. All VLPs lack viral nucleic acid and thus are noninfectious. VLPs have been produced in different eukaryotic and prokaryotic heterologous expression systems:


Examples of nanomaterials constructed by plant cells system viewed using transmission electron microscopyFigure 1. Examples of nanomaterials constructed by plant cells system viewed using transmission electron microscopy.

During the last two decades, plant cell system has been intensely investigated and optimized for recombinant proteins production. Biomass can be generated without expensive fermentation. This system is also proved to have a powerful machinery for post-translational modification and proper assembly of proteins. Particularly, it is capable of producing many VLP products at low cost with little risk of introducing adventitious human pathogens. Plant cells are also very versatile and flexible which can express both enveloped VLPs and non-enveloped VLPs. Of note, the resulting products can be orally delivered without destroying biological activity.

However, it is suggested that in some cases heterologous proteins expressed in planta shows extensive oligomerization for the formation of VLPs, which can lead to relatively low yield. To overcome this issue, we have developed several novel vectors based on “deconstructed” plant viruses to optimize the expression level. Currently, Creative Biolabs has already successfully constructed customized VLPs products using our distinctive plant cells systems such as tobacco plants, potato tubers, and other plants.

Creative Biolabs has established multiple comprehensive platforms to provide customized virus-like particles (VLPs) production services. After years of dedication, we can provide all-round service package from initial construct design, expression, purification (e.g. sucrose gradient, SEC) and characterization (e.g. TEM, DSL). Our core values are driven by a desire to help our clients to achieve scientific excellence, operate with the highest standards of integrity. Please feel free to inquire us for further discussions.

References

  1. Zeltins, A. (2013). Construction and characterization of virus-like particles: a review. Molecular biotechnology, 53(1), 92-107.
  2. Chen Q, Lai H. (2013). Plant-derived virus-like particles as vaccines. Human vaccines & immunotherapeutics, 9(1), 26-49.
  3. Gleba Y, Klimyuk V, Marillonnet S. (2007). Viral vectors for the expression of proteins in plants. Current Opinion in Biotechnology, 18(2), 134-141.
  4. Lai H, Chen Q. (2012). Bioprocessing of plant-derived virus-like particles of Norwalk virus capsid protein under current Good Manufacture Practice regulations. Plant Cell Reports, 31(3), 573-584.



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