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Yeast Display Antibody Library Construction Service

Yeast surface display is a reliable, versatile, and quantitative technique for generating antibodies with tailored properties. It is now widely employed for screening libraries of antibody fragments with high success. Creative Biolabs has a distinguished track record in antibody discovery services, accelerating biopharmaceutical development, and overcoming significant challenges. Our proprietary yeast display antibody library construction service has consistently helped clients discover the antibodies they need.

Services Workflow Advantages Case

Yeast Display Antibody Library Construction Services

At Creative Biolabs, we are committed to facilitating the discovery of high-affinity antibodies for your research and therapeutic needs. Our advanced techniques and expert team ensure the creation of robust, high-quality libraries tailored to your specific targets. Leveraging our efficient and validated Ultraff™ Yeast Display Platform, we provide precise library quality control to sample genetic diversity and verify the integrity and functionality of displayed antibodies.

Workflow and Customization Options

01Ultraff™ Yeast Display Platform

An efficient and validated system of yeast display antibody library construction for novel binding proteins and high-affinity fragments to a specified target.

02Design and Construction

Custom antibody libraries based on your specific requirements, using various sources such as immune libraries from immunized animals, naïve libraries from non-immunized sources, and semi-synthetic or synthetic libraries from designed synthetic DNA sources.

03Library QC and Diversity

Library quality control to sample genetic diversity and verify that most clones are of full length (optional but recommended) to boast high diversity, increasing the likelihood of identifying antibodies with the desired properties.

We offer customized solutions for various formats of antibody libraries tailored to your specific requirements, including but not limited to:


Related Services

We are also proud to provide other yeast display library construction services, including but not limited to:

Yeast Display Non-Antibody Protein Library Construction

Yeast Display Peptide Library Construction

Yeast Display cDNA Library Construction

Why Choose Yeast Display for Antibody Library Construction?

For years, yeast display has been recognized as a robust, versatile, and quantitative method for isolating and engineering antibody fragments. Key advantages include the ease of constructing diverse libraries and the ability to perform quantitative, real-time assessment of candidate clones through flow cytometric analysis.


High Affinity Selection
Yeast display allows for the selection of antibodies with exceptionally high affinity and specificity through iterative rounds of selection (e.g., fluorescence-activated cell sorting, or FACS).

Precise Control
The system enables precise control over expression levels and conditions, ensuring optimal folding and functional display of antibodies.

Functional Screening
Yeast display supports the functional screening of antibodies in a eukaryotic context, closely mimicking natural conditions.

Versatility
It is suitable for a wide range of applications, including therapeutic antibody development, diagnostic tool creation, and basic research.

Cases

Yeast Display scFv Antibody Library Construction

We have designed the library plasmid and constructed an scFv antibody library with a capacity of 107. This library features a 100% insert ratio, with a positive accuracy rate validated at 75% through DNA sequencing. The scFv antibody display efficiency, detected by FACS, exceeds 80%.

Library size 108 Insertion rate 100% Positive Accuracy Rate 75%
Tube Name R3 % Parent CK1 0.02% Display 81.52%

Fig.1 Case for yeast display scFv antibody library construction. (Creative Biolabs Original)

Ready to elevate your research with our yeast display library construction services? Contact us to discuss your requirements and learn how our yeast display antibody library construction services can accelerate your research and development efforts.


All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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