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Yeast Display Library Screening Services

Antibody generation, targeting membrane proteins, screening against challenging targets, generating novel constructs such as ADCs and bispecific are all important applications of yeast screening and display technologies. With extensive experience and advanced technology, Creative Biolabs offers our worldwide clients the best yeast display library screening services through our excellent biopanning strategies and state-of-the-art equipment. Our scientists are willing to tailor the most appropriate strategies to screen the interested libraries of our customers.

Yeast Display Library Screening

Yeast surface display has been widely utilized to screen large libraries for proteins or protein fragments with specific binding properties, and protocols for the construction and application of yeast surface display libraries have been previously described. Yeast antibody display libraries can be coupled with FACS to select high-affinity antibodies that bind specifically to the targeted antigen by using labeled target antigens. Utilization of yeast antibody display combined with FACS allows a more controlled and quantitative selection process that can be fine-tuned to enrich clones with specific binding properties more effectively than phage display.

Methods of Yeast Display Library Screening

The selected yeast cells isolated after the magnetic bead-based processes are further screened using fluorescence-activated cell sorting (FACS) to isolate clones that exhibit highest binding affinity toward the desired target. Quantitative flow cytometric analysis enables fine discrimination of kinetic parameters for protein binding to soluble ligands. A flow cytometry sorting is first performed immediately after the magnetic bead-based processes to ensure selection of yeast cell clones displaying full-length binders. A second flow cytometry sorting is then executed to enrich for higher affinity full-length binders to the target. The yeast display vectors are designed to include HA and c-myc epitope tags at the N-terminus and C-terminus of the yeast surface displayed protein scaffold, allowing for detection and selection of full-length binders by flow cytometry. After the population with the binding of interest is enriched and collected, individual clones of the population are identified by DNA sequencing and characterized using yeast surface titrations to determine the binding affinity (KD).

Yeast Display Library Screening ServicesFig. 1 The protein isolation and engineering process using yeast cell surface display (Angelini, et al., 2015).

Screening Service Available


Applications of Our Yeast Display Library Screening Service

Yeast Display Library Screening Services

Yeast surface display is a powerful tool for library screening and protein engineering especially in the field of antibody discovery and development. With advanced technology platforms and specialized expertise, scientists at Creative Biolabs are ready to serve our clients with high-quality library screening services around the world.

If you are interested in learning more about Creative Biolabs’ yeast display services, please contact us for more details.

Reference

  1. Angelini, A., et al. Protein engineering and selection using yeast surface display. In Yeast Surface Display (pp. 3-36). 2015, Humana Press, New York, NY.

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